The unconjugated antibody was purified by protein A (G) affinity chromatography (>95% by SDS-PAGE). After conjugation, the antibody was purified by size-exclusion chromatography and adjusted for direct use.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Flow Cyt: Use 20µl for 106 cells.
ICC/IF: Use at an assay dependent dilution.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
Cleaves a variety of phosphodiester and phosphosulfate bonds including deoxynucleotides, nucleotide sugars, and NAD.
Expressed in bile ducts, prostate, uterus and colon. Exclusively expressed on basophils, mast cells and their progenitors.
Belongs to the nucleotide pyrophosphatase/phosphodiesterase family. Contains 2 SMB (somatomedin-B) domains.
N-glycosylation is necessary for correct trafficking to the apical surface, but is not the apical targeting signal. It has been suggested that the active SMB domain may be permitted considerable disulfide bond heterogeneity or variability, thus two alternate disulfide patterns based on 3D structures are described with 1 disulfide bond conserved in both.
Membrane. Secreted. Located to the apical surface in intestinal and kidney epithelial cells. Located to the cell surface of basophils, and to the apical plasma membrane of bile duct cells. Secreted in serum, and in lumen of epithelial cells.
Surface staining of activated human basophil by FITC-conjugated anti-CD63 (clone MEM-259, ab18235, green) and PE-conjugated anti-ENPP3 (clone NP4D6, ab90751, red); merged signal yellow. DNA visualized by DAPI (blue).