纯化说明The unconjugated antibody was purified by protein A (G) affinity chromatography (>95% by SDS-PAGE). After conjugation, the antibody was purified by size-exclusion chromatography and adjusted for direct use.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
应用说明Flow Cyt: Use 20µl for 106 cells.
ICC/IF: Use at an assay dependent dilution.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
功能Cleaves a variety of phosphodiester and phosphosulfate bonds including deoxynucleotides, nucleotide sugars, and NAD.
组织特异性Expressed in bile ducts, prostate, uterus and colon. Exclusively expressed on basophils, mast cells and their progenitors.
序列相似性Belongs to the nucleotide pyrophosphatase/phosphodiesterase family. Contains 2 SMB (somatomedin-B) domains.
翻译后修饰N-glycosylation is necessary for correct trafficking to the apical surface, but is not the apical targeting signal. It has been suggested that the active SMB domain may be permitted considerable disulfide bond heterogeneity or variability, thus two alternate disulfide patterns based on 3D structures are described with 1 disulfide bond conserved in both.
细胞定位Membrane. Secreted. Located to the apical surface in intestinal and kidney epithelial cells. Located to the cell surface of basophils, and to the apical plasma membrane of bile duct cells. Secreted in serum, and in lumen of epithelial cells.
Surface staining of activated human basophil by FITC-conjugated anti-CD63 (clone MEM-259, ab18235, green) and PE-conjugated anti-ENPP3 (clone NP4D6, ab90751, red); merged signal yellow. DNA visualized by DAPI (blue).