概述

  • 产品名称Anti-ENOX2抗体
    参阅全部 ENOX2 一抗
  • 描述
    山羊多克隆抗体to ENOX2
  • 特异性ab77482 is expected to recognise both reported isoforms (NP_006366.2; NP_872114.1).
  • 经测试应用适用于: ELISA, IHC-Pmore details
  • 种属反应性

    预测可用于: Cow, Dog, Human, Rhesus monkey
  • 免疫原

    Synthetic peptide:

    C-EKLKDDKLQVEK

    , from an internal region of Human ENOX2 (NP_006366.2; NP_872114.1).

  • 阳性对照
    • Human Heart lysate.

性能

  • 形式Liquid
  • 存放说明Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
  • 存储溶液Preservative: 0.02% Sodium Azide
    Constituents: 0.5% BSA, Tris saline, pH 7.3
  • Concentration information loading...
  • 纯度Immunogen affinity purified
  • 纯化说明ab77482 is purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunising peptide.
  • 克隆多克隆
  • 同种型IgG
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab77482 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ELISA
IHC-P
  • 应用说明Peptide ELISA: Antibody detection limit dilution 1/64000.
    WB: Preliminary experiments gave bands at approx 60kDa and 37kDa in Human Heart lysates after 0.03µg/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the bands we observe given the calculated size of 66.6kDa and 70.1kDa according to NP_006366.2 and NP_872114.1. Both detected bands were successfully blocked by incubation with the immunising peptide (and BLAST results with the immunising peptide sequence did not identify any other proteins to explain the additional bands).


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • 靶标

    • 功能May be involved in cell growth. Probably acts as a terminal oxidase of plasma electron transport from cytosolic NAD(P)H via hydroquinones to acceptors at the cell surface. Hydroquinone oxidase activity alternates with a protein disulfide-thiol interchange/oxidoreductase activity which may control physical membrane displacements associated with vesicle budding or cell enlargement. The activities oscillate with a period length of 22 minutes and play a role in control of the ultradian cellular biological clock.
    • 组织特异性Found in the sera of cancer patients with a wide variety of cancers including breast, prostate, lung and ovarian cancers, leukemias, and lymphomas. Not found in the serum of healthy volunteers or patients with disorders other than cancer. Probably shed into serum by cancer cells. Found on the cell borders of renal, kidney and ovarian carcinomas but not on the borders of surrounding non-cancerous stromal cells.
    • 序列相似性Belongs to the ENOX family.
      Contains 1 RRM (RNA recognition motif) domain.
    • 翻译后修饰Glycosylated.
    • 细胞定位Cell membrane. Secreted > extracellular space. Extracellular and plasma membrane-associated.
    • Information by UniProt
    • 数据库链接
    • 别名
      • APK1 antibody
      • APK1 antigen antibody
      • COVA1 antibody
      • Cytosolic ovarian carcinoma antigen 1 antibody
      • Ecto NOX disulfide thiol exchanger 2 antibody
      • ENOX 2 antibody
      • ENOX2 antibody
      • ENOX2_HUMAN antibody
      • OTTHUMP00000024021 antibody
      • OTTHUMP00000024022 antibody
      • Protein disulfide-thiol oxidoreductase antibody
      • tNOX antibody
      • Tumor associated hydroquinone oxidase antibody
      • Tumor-associated hydroquinone oxidase antibody
      see all

    Anti-ENOX2 antibody 图像

    • Immunohistochemistry (paraffin-embedded sections) analysis of human kidney labeling ENOX2 with ab77482 at 4µg/ml. Microwaved antigen retrieval was performed using Tris/EDTA buffer pH9. HRP-staining.

    Anti-ENOX2 antibody (ab77482)参考文献

    ab77482 has not yet been referenced specifically in any publications.

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"