概述

  • 产品名称Anti-ENOX1抗体
    参阅全部 ENOX1 一抗
  • 描述
    兔多克隆抗体to ENOX1
  • 经测试应用适用于: WB, ELISAmore details
  • 种属反应性
    与反应: Human
    预测可用于: Mouse, Rat, Rabbit, Horse, Chicken, Guinea pig, Cow, Cat, Dog
  • 免疫原

    Synthetic peptide from within the internal sequence amino acids 468 - 517: QQLQFLQQTM QGMQQQLLTI QEELNNKKSE LEQAKEEQSH TQALLKVLQE, of Human ENOX1 (NP_060463)

  • 阳性对照
    • 721_B cell lysate

性能

  • 形式Liquid
  • 存放说明Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
  • 存储溶液Preservative: None
    Constituents: 2% Sucrose, PBS
  • Concentration information loading...
  • 纯度Immunogen affinity purified
  • 纯化说明ab83540 is purified by a peptide affinity chromatography method.
  • 克隆多克隆
  • 同种型IgG
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab83540 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 73 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
ELISA Use at an assay dependent concentration.

ELISA titre using peptide based assay: 1/312500.

靶标

  • 功能Probably acts as a terminal oxidase of plasma electron transport from cytosolic NAD(P)H via hydroquinones to acceptors at the cell surface. Hydroquinone oxidase activity alternates with a protein disulfide-thiol interchange/oxidoreductase activity which may control physical membrane displacements associated with vesicle budding or cell enlargement. The activities oscillate with a period length of 24 minutes and play a role in control of the ultradian cellular biological clock.
  • 组织特异性Expressed in lymphocyte cells, breast and breast cancer (at protein level). Found in the sera of cancer patients with a wide variety of cancers including breast, prostate, lung and ovarian cancers, leukemias, and lymphomas. Found also in the serum of healthy volunteers or patients with disorders other than cancer. Probably shed into serum by cancer cells.
  • 序列相似性Belongs to the ENOX family.
    Contains 1 RRM (RNA recognition motif) domain.
  • 细胞定位Cell membrane. Secreted > extracellular space. Extracellular and plasma membrane-associated.
  • Information by UniProt
  • 数据库链接
  • 别名
    • bA64J21.1 antibody
    • candidate growth related and time keeping constitutive hydroquinone (NADH) oxidase antibody
    • Candidate growth related and time keeping constitutive hydroquinone [NADH] oxidase antibody
    • Candidate growth-related and time keeping constitutive hydroquinone [NADH] oxidase antibody
    • cCNOX antibody
    • Cell proliferation inducing gene 38 protein antibody
    • Cell proliferation-inducing gene 38 protein antibody
    • cNOX antibody
    • Constitutive Ecto NOX antibody
    • Constitutive Ecto-NOX antibody
    • Ecto NOX disulfide thiol exchanger 1 antibody
    • ENOX1 antibody
    • ENOX1_HUMAN antibody
    • FLJ10094 antibody
    • OTTHUMP00000018332 antibody
    • PIG38 antibody
    • Proliferation inducing protein 38 antibody
    • Protein disulfide-thiol oxidoreductase antibody
    see all

Anti-ENOX1 antibody 图像

  • Anti-ENOX1 antibody (ab83540) at 1 µg/ml (in 5% skim milk / PBS buffer) + 721_B cell lysate at 10 µg

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size : 73 kDa
    Observed band size : 63 kDa (why is the actual band size different from the predicted?)

Anti-ENOX1 antibody (ab83540)参考文献

ab83540 has not yet been referenced specifically in any publications.

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