Anti-eNOS (phospho S1177)抗体(ab51038)


  • 产品名称Anti-eNOS (phospho S1177)抗体
    参阅全部 eNOS 一抗
  • 描述
    兔多克隆抗体to eNOS (phospho S1177)
  • 特异性This antibody detects endogenous levels of eNOS only when phosphorylated at serine 1176
  • 经测试应用适用于: WB, ELISAmore details
  • 种属反应性
    与反应: Human
    预测可用于: Mouse, Rat
  • 免疫原

    Synthetic peptide corresponding to Human eNOS (phospho S1177).

  • 阳性对照
    • HeLa cell extract



Our Abpromise guarantee covers the use of ab51038 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/500 - 1/1000. Detects a band of approximately 133 kDa (predicted molecular weight: 133 kDa).
ELISA 1/20000.


  • 功能Produces nitric oxide (NO) which is implicated in vascular smooth muscle relaxation through a cGMP-mediated signal transduction pathway. NO mediates vascular endothelial growth factor (VEGF)-induced angiogenesis in coronary vessels and promotes blood clotting through the activation of platelets.
    Isoform eNOS13C: Lacks eNOS activity, dominant-negative form that may down-regulate eNOS activity by forming heterodimers with isoform 1.
  • 组织特异性Platelets, placenta, liver and kidney.
  • 疾病相关Variation in NOS3 seem to be associated with susceptibility to coronary spasm.
  • 序列相似性Belongs to the NOS family.
    Contains 1 FAD-binding FR-type domain.
    Contains 1 flavodoxin-like domain.
  • 翻译后修饰Phosphorylation by AMPK at Ser-1177 in the presence of Ca(2+)-calmodulin (CaM) activates activity. In absence of Ca(2+)-calmodulin, AMPK also phosphorylates Thr-495, resulting in inhibition of activity (By similarity). Phosphorylation of Ser-114 by CDK5 reduces activity.
  • 细胞定位Cell membrane. Membrane, caveola. Cytoplasm, cytoskeleton. Golgi apparatus. Specifically associates with actin cytoskeleton in the G2 phase of the cell cycle and which is favored by interaction with NOSIP and results in a reduced enzymatic activity.
  • Information by UniProt
  • 数据库链接
  • 别名
    • cNOS antibody
    • Constitutive NOS antibody
    • EC NOS antibody
    • EC-NOS antibody
    • ecNOS antibody
    • Endothelial nitric oxidase synthase antibody
    • Endothelial nitric oxide synthase antibody
    • Endothelial nitric oxide synthase 3 antibody
    • Endothelial NOS antibody
    • eNOS antibody
    • Nitric oxide synthase 3 (endothelial cell) antibody
    • Nitric oxide synthase 3 antibody
    • Nitric oxide synthase 3 endothelial cell antibody
    • Nitric oxide synthase endothelial antibody
    • Nitric oxide synthase, endothelial antibody
    • NOS 3 antibody
    • NOS III antibody
    • NOS type III antibody
    • NOS3 antibody
    • NOS3_HUMAN antibody
    • NOSIII antibody
    see all

Anti-eNOS (phospho S1177) antibody 图像

  • All lanes : Anti-eNOS (phospho S1177) antibody (ab51038) at 1/500 dilution

    Lane 1 : HeLa cell extract treated with Insulin (0.01 U/ml for 15min), with the immunising peptide
    Lane 2 : HeLa cell extract treated with Insulin (0.01 U/ml for 15min), with the immunising peptide

    Predicted band size : 133 kDa
    Observed band size : 133 kDa

Anti-eNOS (phospho S1177) antibody (ab51038)参考文献

This product has been referenced in:
  • Cheang WS  et al. Endothelial nitric oxide synthase enhancer reduces oxidative stress and restores endothelial function in db/db mice. Cardiovasc Res 92:267-75 (2011). Read more (PubMed: 21875904) »

See 1 Publication for this product

Product Wall

Thank you for your patience. I managed to locate a publication that used endothelia from aorta of rats for an eNOS (phospho S1176) experiment. Therefore, this can also be used as a positive control for ab51038. Please see the Materials and Meth...

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Thank you for your enquiry. I can see from the SwissProt database entry for this protein ( that they list amino acid 1177 as being a phosphoserine. If you look at the sequence, however, they are incl...

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