概述

  • 产品名称
    Anti-eNOS (phospho S116)抗体
    参阅全部 eNOS 一抗
  • 描述
    兔多克隆抗体to eNOS (phospho S116)
  • 特异性
    ab78004 recognizes eNOS phosphorylated on serine 116
  • 经测试应用
    适用于: WBmore details
  • 种属反应性
    与反应: Cow, Human
    预测可用于: Sheep, Pig
  • 免疫原

    Synthetic peptide: PRKLQGRPSPPGPP-C conjugated to KLH, corresponding to amino acids 106-118 of Human eNOS

  • 阳性对照
    • Fetal bovine heart endothelial cell lysate.

性能

应用

Our Abpromise guarantee covers the use of ab78004 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/500 - 1/1000. Detects a band of approximately 133 kDa (predicted molecular weight: 133 kDa).

靶标

  • 功能
    Produces nitric oxide (NO) which is implicated in vascular smooth muscle relaxation through a cGMP-mediated signal transduction pathway. NO mediates vascular endothelial growth factor (VEGF)-induced angiogenesis in coronary vessels and promotes blood clotting through the activation of platelets.
    Isoform eNOS13C: Lacks eNOS activity, dominant-negative form that may down-regulate eNOS activity by forming heterodimers with isoform 1.
  • 组织特异性
    Platelets, placenta, liver and kidney.
  • 疾病相关
    Variation in NOS3 seem to be associated with susceptibility to coronary spasm.
  • 序列相似性
    Belongs to the NOS family.
    Contains 1 FAD-binding FR-type domain.
    Contains 1 flavodoxin-like domain.
  • 翻译后修饰
    Phosphorylation by AMPK at Ser-1177 in the presence of Ca(2+)-calmodulin (CaM) activates activity. In absence of Ca(2+)-calmodulin, AMPK also phosphorylates Thr-495, resulting in inhibition of activity (By similarity). Phosphorylation of Ser-114 by CDK5 reduces activity.
  • 细胞定位
    Cell membrane. Membrane, caveola. Cytoplasm, cytoskeleton. Golgi apparatus. Specifically associates with actin cytoskeleton in the G2 phase of the cell cycle and which is favored by interaction with NOSIP and results in a reduced enzymatic activity.
  • Information by UniProt
  • 数据库链接
  • 别名
    • cNOS antibody
    • Constitutive NOS antibody
    • EC NOS antibody
    • EC-NOS antibody
    • ecNOS antibody
    • Endothelial nitric oxidase synthase antibody
    • Endothelial nitric oxide synthase antibody
    • Endothelial nitric oxide synthase 3 antibody
    • Endothelial NOS antibody
    • eNOS antibody
    • Nitric oxide synthase 3 (endothelial cell) antibody
    • Nitric oxide synthase 3 antibody
    • Nitric oxide synthase 3 endothelial cell antibody
    • Nitric oxide synthase endothelial antibody
    • Nitric oxide synthase, endothelial antibody
    • NOS 3 antibody
    • NOS III antibody
    • NOS type III antibody
    • NOS3 antibody
    • NOS3_HUMAN antibody
    • NOSIII antibody
    see all

图片

  • All lanes : Anti-eNOS (phospho S116) antibody (ab78004) at 1/1000 dilution

    Lane 1 : Fetal bovine heart endothelial cell lysate
    Lane 2 : Lambda phosphatase-treated fetal bovine heart endothelial cell lysate

    Secondary
    Goat anti-rabbit HRP conjugate

    Predicted band size : 133 kDa
    Observed band size : 133 kDa
    Additional bands at : 190 kDa. We are unsure as to the identity of these extra bands.

实验方案

文献

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