概述

  • 产品名称Anti-eNOS抗体
    参阅全部 eNOS 一抗
  • 描述
    兔多克隆抗体to eNOS
  • 特异性This antibody is specific for nitric oxide synthase (NOS), derived from endothelial tissue, but does not recognize NOS derived from brain (bNOS) and macrophages (iNOS).
  • 经测试应用适用于: WB, IHC-Fr, IHC-FoFrmore details
  • 种属反应性
    与反应: Mouse, Cow
    预测可用于: Rat, Guinea pig, Human
  • 免疫原

    Synthetic peptide:

    SGPYNSSPRPEQHK

    conjugated to KLH, corresponding to internal sequence amino acids 596-609 of Cow eNOS

  • 阳性对照
    • Whole extract of bovine lung endothelial cells or mouse heart tissue sections.

性能

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应用

Our Abpromise guarantee covers the use of ab50260 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/10000. Predicted molecular weight: 133 kDa.
IHC-Fr 1/100. Fix with acetone.
IHC-FoFr Use at an assay dependent concentration. PubMed: 20702812

靶标

  • 功能Produces nitric oxide (NO) which is implicated in vascular smooth muscle relaxation through a cGMP-mediated signal transduction pathway. NO mediates vascular endothelial growth factor (VEGF)-induced angiogenesis in coronary vessels and promotes blood clotting through the activation of platelets.
    Isoform eNOS13C: Lacks eNOS activity, dominant-negative form that may down-regulate eNOS activity by forming heterodimers with isoform 1.
  • 组织特异性Platelets, placenta, liver and kidney.
  • 疾病相关Variation in NOS3 seem to be associated with susceptibility to coronary spasm.
  • 序列相似性Belongs to the NOS family.
    Contains 1 FAD-binding FR-type domain.
    Contains 1 flavodoxin-like domain.
  • 翻译后修饰Phosphorylation by AMPK at Ser-1177 in the presence of Ca(2+)-calmodulin (CaM) activates activity. In absence of Ca(2+)-calmodulin, AMPK also phosphorylates Thr-495, resulting in inhibition of activity (By similarity). Phosphorylation of Ser-114 by CDK5 reduces activity.
  • 细胞定位Cell membrane. Membrane, caveola. Cytoplasm, cytoskeleton. Golgi apparatus. Specifically associates with actin cytoskeleton in the G2 phase of the cell cycle and which is favored by interaction with NOSIP and results in a reduced enzymatic activity.
  • Information by UniProt
  • 数据库链接
  • 别名
    • cNOS antibody
    • Constitutive NOS antibody
    • EC NOS antibody
    • EC-NOS antibody
    • ecNOS antibody
    • Endothelial nitric oxidase synthase antibody
    • Endothelial nitric oxide synthase antibody
    • Endothelial nitric oxide synthase 3 antibody
    • Endothelial NOS antibody
    • eNOS antibody
    • Nitric oxide synthase 3 (endothelial cell) antibody
    • Nitric oxide synthase 3 antibody
    • Nitric oxide synthase 3 endothelial cell antibody
    • Nitric oxide synthase endothelial antibody
    • Nitric oxide synthase, endothelial antibody
    • NOS 3 antibody
    • NOS III antibody
    • NOS type III antibody
    • NOS3 antibody
    • NOS3_HUMAN antibody
    • NOSIII antibody
    see all

Anti-eNOS antibody (ab50260)参考文献

This product has been referenced in:
  • Feng J  et al. Endothelium-dependent coronary vasodilatation requires NADPH oxidase-derived reactive oxygen species. Arterioscler Thromb Vasc Biol 30:1703-10 (2010). IHC-FoFr ; Mouse . Read more (PubMed: 20702812) »

See 1 Publication for this product

Product Wall

Application Immunohistochemistry (Frozen sections)
Sample Rat Tissue sections (Mesentery sections)
Permeabilization Yes - Triton X-100
Specification Mesentery sections
Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 37°C
Fixative Acetone
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提交于 Nov 24 2015

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"