概述

  • 产品名称Anti-eNOS抗体
    参阅全部 eNOS 一抗
  • 描述
    兔多克隆抗体to eNOS
  • 经测试应用适用于: WB, IHC-Pmore details
  • 种属反应性
    与反应: Human
    预测可用于: Mouse, Rat, Guinea pig, Cow, Dog, Pig
  • 免疫原

    Synthetic peptide:

    QERQLRGAVPWAFD

    conjugated to KLH, corresponding to C terminal amino acids 1181-1194 of Human eNOS (NP_000594.2).

  • 阳性对照
    • Human Placenta and Spleen tissue; HUVEC cell lysate.
  • 常规说明Aliquots are stable for at least 12 months at -20°C.

性能

应用

Our Abpromise guarantee covers the use of ab119292 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use a concentration of 0.5 - 2 µg/ml. Predicted molecular weight: 133 kDa.
IHC-P Use a concentration of 5 µg/ml.

靶标

  • 功能Produces nitric oxide (NO) which is implicated in vascular smooth muscle relaxation through a cGMP-mediated signal transduction pathway. NO mediates vascular endothelial growth factor (VEGF)-induced angiogenesis in coronary vessels and promotes blood clotting through the activation of platelets.
    Isoform eNOS13C: Lacks eNOS activity, dominant-negative form that may down-regulate eNOS activity by forming heterodimers with isoform 1.
  • 组织特异性Platelets, placenta, liver and kidney.
  • 疾病相关Variation in NOS3 seem to be associated with susceptibility to coronary spasm.
  • 序列相似性Belongs to the NOS family.
    Contains 1 FAD-binding FR-type domain.
    Contains 1 flavodoxin-like domain.
  • 翻译后修饰Phosphorylation by AMPK at Ser-1177 in the presence of Ca(2+)-calmodulin (CaM) activates activity. In absence of Ca(2+)-calmodulin, AMPK also phosphorylates Thr-495, resulting in inhibition of activity (By similarity). Phosphorylation of Ser-114 by CDK5 reduces activity.
  • 细胞定位Cell membrane. Membrane, caveola. Cytoplasm, cytoskeleton. Golgi apparatus. Specifically associates with actin cytoskeleton in the G2 phase of the cell cycle and which is favored by interaction with NOSIP and results in a reduced enzymatic activity.
  • Information by UniProt
  • 数据库链接
  • 别名
    • cNOS antibody
    • Constitutive NOS antibody
    • EC NOS antibody
    • EC-NOS antibody
    • ecNOS antibody
    • Endothelial nitric oxidase synthase antibody
    • Endothelial nitric oxide synthase antibody
    • Endothelial nitric oxide synthase 3 antibody
    • Endothelial NOS antibody
    • eNOS antibody
    • Nitric oxide synthase 3 (endothelial cell) antibody
    • Nitric oxide synthase 3 antibody
    • Nitric oxide synthase 3 endothelial cell antibody
    • Nitric oxide synthase endothelial antibody
    • Nitric oxide synthase, endothelial antibody
    • NOS 3 antibody
    • NOS III antibody
    • NOS type III antibody
    • NOS3 antibody
    • NOS3_HUMAN antibody
    • NOSIII antibody
    see all

Anti-eNOS antibody 图像

  • ab119292, at 5 µg/ml, staining eNOS in formalin-fixed, paraffin-embedded Human Placenta tissue by Immunohistochemistry.
  • ab119292, at 5 µg/ml, staining eNOS in formalin-fixed, paraffin-embedded Human Spleen tissue by Immunohistochemistry.

Anti-eNOS antibody (ab119292)参考文献

ab119292 has not yet been referenced specifically in any publications.

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