概述

  • 产品名称ENO1人Activity Assay试剂盒
  • 检测方法Colorimetric
  • 精确度
    批次内
    样品 n Mean SD CV%
    Cell extract 9 9%
    批次间
    样品 n Mean SD CV%
    Cell extract 4 11%
  • 测试
    1 x 96 well plate
  • 样品类型
    Cell culture extracts, Tissue Extracts
  • 检测类型Enzyme activity
  • 灵敏度
    125 ng/ml
  • 范围
    125 ng/ml - 2000 ng/ml
  • 复检

    90 %

    特定样本复检结果
    样品类型 平均% 范围
    Cell culture supernatant 63 % - %
    Serum 140 % - %

  • 实验步骤Multiple steps standard assay
  • 种属反应性
    与反应: Human
    不与反应: Mouse, Rat
  • 产品概述

    ab117994 is used to determine enolase 1 (ENO1) activity in a sample. The native enzyme is immunocaptured within the wells of the microplate; this removes all other enzymes. The enolase activity is determined via a coupled reaction to the consumption of NADH in an assay buffer.

  • 经测试应用适用于: Functional Studiesmore details
  • 平台Microplate

性能

应用

Our Abpromise guarantee covers the use of ab117994 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
Functional Studies Use at an assay dependent concentration.

ENO1 Human Activity Assay Kit 图像

  • Analysis of recombinant ENO1 (ab89248) and the neuron-specific recombinant ENO2 (ab78797), each at 2000 ng/mL. The relative ENO activity was determined from HepG2 standard curve as described in the protocol. The data show that the activity of the neuron-specific ENO2 can’t be detected.
  • Example of dilution series prepared from HepG2 cells extract.
  • Immunofluorescent detection of ENO1 in cytosol in HeLa cells using ab112994.
  • Scheme of reactions used in this kit. Abbreviations are as follows: enolase (ENO), 2-phospho-D-glycerate (2PG), phosphoenolpyruvate (PEP), pyruvate kinase (PK), lactate dehydrogenase (LDH).

实验方案

ENO1 Human Activity Assay Kit (ab117994)参考文献

This product has been referenced in:
  • Bottermann K  et al. Systematic Analysis Reveals Elongation Factor 2 and a-Enolase as Novel Interaction Partners of AKT2. PLoS One 8:e66045 (2013). Read more (PubMed: 23823123) »

See 1 Publication for this product

Product Wall

Regarding LDH control:
Please note that the LDH is simply added to the rehydrated 'Reagent Mix', along with PK, to produce the 1x Acitvity Solution.

Regarding data analysis:
If data collected in a kinetic mode, they are presented as...

Read More

Vielen Dank für Ihren Anruf.

Bitte entschuldigen Sie die verspätete Antwort. MitoSciences sitzt an der Westküste in den USA und durch die Zeitverschiebung habe ich erst jetzt eine Antwort erhalten.

Ich freue mich Ihne...

Read More

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"