概述

  • 产品名称Anti-Enkephalin抗体
    参阅全部 Enkephalin 一抗
  • 描述
    山羊多克隆抗体to Enkephalin
  • 经测试应用适用于: WB, IHC-FoFrmore details
  • 种属反应性
    与反应: Human
    预测可用于: Chimpanzee
  • 免疫原

    Synthetic peptide:

    RSHHQDGSDNEE

    , corresponding to internal sequence amino acids 169-180 of Human Enkephalin

  • 阳性对照
    • Human Adrenal Gland lysate

性能

  • 形式Liquid
  • 存放说明Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • 存储溶液Preservative: 0.02% Sodium Azide
    Constituents: 0.5% BSA, Tris buffered saline, pH 7.3
  • Concentration information loading...
  • 纯度Immunogen affinity purified
  • 纯化说明Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
  • 克隆多克隆
  • 同种型IgG
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab77273 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use a concentration of 1 - 3 µg/ml. Detects a band of approximately 37 kDa (predicted molecular weight: 31 kDa).
IHC-FoFr Use a concentration of 0.3 - 1 µg/ml.

靶标

  • 功能Met- and Leu-enkephalins compete with and mimic the effects of opiate drugs. They play a role in a number of physiologic functions, including pain perception and responses to stress. PENK(114-133) and PENK(237-258) increase glutamate release in the striatum. PENK(114-133) decreases GABA concentration in the striatum.
  • 序列相似性Belongs to the opioid neuropeptide precursor family.
  • 翻译后修饰The N-terminal domain contains 6 conserved cysteines thought to be involved in disulfide bonding and/or processing.
  • 细胞定位Secreted.
  • Information by UniProt
  • 数据库链接
  • 别名
    • Enkephalin A antibody
    • Met-enkephalin-Arg-Phe antibody
    • OGF antibody
    • Opioid growth factor antibody
    • PENK antibody
    • PENK_HUMAN antibody
    • Preproenkephalin antibody
    • Proenkephalin A antibody
    • Proenkephalin antibody
    see all

Anti-Enkephalin antibody 图像

  • Anti-Enkephalin antibody (ab77273) at 0.3 µg/ml + Human Adrenal Gland lysate in RIPA buffer at 35 µg

    Predicted band size : 31 kDa
    Observed band size : 37 kDa (why is the actual band size different from the predicted?)
  • Immunohistochemical analysis of PFA-perfused frozen Human hypothalamus tissue, staining Enkephalin with ab77273 at 0.3 µg/ml.

Anti-Enkephalin antibody (ab77273)参考文献

ab77273 has not yet been referenced specifically in any publications.

Product Wall

There are currently no Abreviews or Questions for ab77273.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"