Anti-Engrailed and Invected抗体[4D9] (ab12454)

概述

  • 产品名称Anti-Engrailed and Invected抗体[4D9]
  • 描述
    小鼠单克隆抗体[4D9] to Engrailed and Invected
  • 特异性This antibody recognizes both the engrailed and invected gene products of Drosophila. The antibody is useful for examining the patterning of segmentation from the cellular blastoderm stage onward. Staining is also seen in a large number of CNS neurons including most of the median neuroblast progeny as well as a small number of PNS neurons.
  • 经测试应用适用于: IHC-FoFrmore details
  • 种属反应性
    与反应: Chicken, Xenopus laevis, Drosophila melanogaster, Zebrafish, Crayfish
    不与反应: Mouse
  • 免疫原

    C-terminal two-thirds of the invected protein were generated in E. coli with the T7 polymerase expression system.

  • 表位Epitope has been localized to residues 38-58 of the homeodomain.

性能

应用

Our Abpromise guarantee covers the use of ab12454 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
IHC-FoFr 1/200. Fix with 4% paraformaldehyde in PBS for 1 hour.

靶标

  • 相关性In Drosophila, the Engrailed gene plays an important role during development in segmentation, where it is required for the formation of posterior compartments. Expression studies have suggested that Engrailed may also function early in organizing the preblastoderm and later in neurogenesis. Engrailed encodes a homeodomain-containing protein that can function as a transcription factor. Invected is homologous to Engrailed and the gene lies adjacent to that for Engrailed. The function of Invected is unclear.
  • 数据库链接
    • 别名
      • En antibody
      • Eng antibody
      • Engrailed antibody
      • Engrailed/Invected antibody
      • Homeobox protein invected antibody
      • Inv antibody
      • Invected antibody
      see all

    Anti-Engrailed and Invected antibody [4D9] 图像

    • ab12454 used in immunohistochemistry at 1/200 on Drosophila embryos. The protocol used is available in the Neuroscience Abwire in the protocols section.

      Briefly, embryos were fixed with PBS, formaldehyde and n-heptane. Embryos were then dechorionated with 100% bleach and rehydrated with PBT (PBS with 0.3% Tritone added) before antibody staining.Visualisation was with DAB.

    • ab12454 used in immunofluorescence at 1/200 on Drosophila embryos. The protocol used is available in the Neuroscience Abwire in the protocols section.

      Briefly, embryos were fixed with PBS, formaldehyde and n-heptane. Embryos were then dechorionated with 100% bleach and rehydrated with PBT (PBS with 0.3% Tritone added) before antibody staining.

    Anti-Engrailed and Invected antibody [4D9] (ab12454)参考文献

    This product has been referenced in:
    • Baratte S  et al. Engrailed in cephalopods: a key gene related to the emergence of morphological novelties. Dev Genes Evol 217:353-62 (2007). Read more (PubMed: 17394016) »
    • Harzsch S Evolution of identified arthropod neurons: the serotonergic system in relation to engrailed-expressing cells in the embryonic ventral nerve cord of the american lobster homarus americanus milne edwards, 1873 (malacostraca, pleocyemata, homarida). Dev Biol 258:44-56 (2003). Read more (PubMed: 12781681) »

    See all 11 Publications for this product

    Product Wall

    Application Immunocytochemistry/ Immunofluorescence
    Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 22°C
    Sample Fruit fly (Drosophila melanogaster) Cell (Drosophila Wing Imaginal disc)
    Specification Drosophila Wing Imaginal disc
    Permeabilization Yes - 0.5% Triton X100
    Fixative Paraformaldehyde
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    提交于 Mar 16 2015

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"