Anti-Endothelin B Receptor抗体(ab39960)

概述

  • 产品名称Anti-Endothelin B Receptor抗体
    参阅全部 Endothelin B Receptor 一抗
  • 描述
    兔多克隆抗体to Endothelin B Receptor
  • 经测试应用适用于: IHC-Fr, IHC-P, ICC/IF, WBmore details
  • 种属反应性
    与反应: Human
    预测可用于: Dog
  • 免疫原

    Synthetic peptide conjugated to KLH derived from within residues 1 - 100 of Human Endothelin B Receptor.

    (Peptide available as ab45351.)

  • 阳性对照
    • This antibody gave a positive result in the following lysates: Liver (Human) Membrane Lysate - adult normal tissue Placenta (Human) Tissue Lysate

性能

  • 形式Liquid
  • 存放说明Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • 存储溶液Preservative: 0.02% Sodium Azide
    Constituents: 1% BSA, PBS, pH 7.4
  • Concentration information loading...
  • 纯度Immunogen affinity purified
  • 克隆多克隆
  • 同种型IgG
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab39960 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
IHC-Fr Use at an assay dependent concentration. PubMed: 21203549
IHC-P 1/200. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
ICC/IF Use a concentration of 1 µg/ml.
WB Use a concentration of 1 µg/ml. Detects a band of approximately 50 kDa (predicted molecular weight: 50 kDa).

靶标

  • 功能Non-specific receptor for endothelin 1, 2, and 3. Mediates its action by association with G proteins that activate a phosphatidylinositol-calcium second messenger system.
  • 组织特异性Expressed in placental stem villi vessels, but not in cultured placental villi smooth muscle cells.
  • 疾病相关Defects in EDNRB are a cause of Waardenburg syndrome type 4A (WS4A) [MIM:277580]; also known as Waardenburg-Shah syndrome. WS4A is characterized by the association of Waardenburg features (depigmentation and deafness) and the absence of enteric ganglia in the distal part of the intestine (Hirschsprung disease).
    Defects in EDNRB are the cause of Hirschsprung disease type 2 (HSCR2) [MIM:600155]; also known as aganglionic megacolon (MGC). HSCR2 is a congenital disorder characterized by absence of enteric ganglia along a variable length of the intestine. It is the most common cause of congenital intestinal obstruction. Early symptoms range from complete acute neonatal obstruction, characterized by vomiting, abdominal distention and failure to pass stool, to chronic constipation in the older child.
    Defects in EDNRB are the cause of ABCD syndrome (ABCDS) [MIM:600501]. ABCD syndrome is an autosomal recessive syndrome characterized by albinism, black lock at temporal occipital region, bilateral deafness, aganglionosis of the large intestine and total absence of neurocytes and nerve fibers in the small intestine.
  • 序列相似性Belongs to the G-protein coupled receptor 1 family. Endothelin receptor subfamily. EDNRB sub-subfamily.
  • 翻译后修饰Palmitoylation of Cys-402 was confirmed by the palmitoylation of Cys-402 in a deletion mutant lacking both Cys-403 and Cys-405.
  • 细胞定位Cell membrane.
  • Information by UniProt
  • 数据库链接
  • 别名
    • ABCDS antibody
    • Ednra antibody
    • EDNRB antibody
    • EDNRB_HUMAN antibody
    • Endothelin B receptor antibody
    • Endothelin B receptor precursor antibody
    • Endothelin receptor Non selective type antibody
    • Endothelin receptor non-selective type antibody
    • Endothelin receptor type B antibody
    • ET B antibody
    • ET-B antibody
    • ET-BR antibody
    • ETB antibody
    • ETBR antibody
    • ETRB antibody
    • Hirschsprung disease 2 antibody
    • HSCR antibody
    • HSCR2 antibody
    • OTTHUMP00000018534 antibody
    • OTTHUMP00000178736 antibody
    • WS4A antibody
    see all

Anti-Endothelin B Receptor antibody 图像

  • ICC/IF image of ab39960 stained human HeLa cells. The cells were PFA fixed (10 min), permabilised in TBS-T (20 min) and incubated with the antibody (ab39960, 1µg/ml) for 1h at room temperature. 1%BSA / 10% normal serum / 0.3M glycine was used to quench autofluorescence and block non-specific protein-protein interactions. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red). DAPI was used to stain the cell nuclei (blue).
  • All lanes : Anti-Endothelin B Receptor antibody (ab39960) at 1 µg/ml

    Lane 1 : MCF7 (Human breast adenocarcinoma cell line) Whole Cell Lysate
    Lane 2 : MDA MB 361 (Human breast adenocarcinoma cell line) Whole Cell Lysate
    Lane 3 : SW480 (Human colon adenocarcinoma cell line) Whole Cell Lysate
    Lane 4 : LOVO (Human colon adenocarcinoma cell line) Whole Cell Lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution

    Performed under reducing conditions.

    Predicted band size : 50 kDa
    Observed band size : 49 kDa (why is the actual band size different from the predicted?)


    Exposure time : 3 minutes
  • ab39960 staining human placenta sections in IHC-P. The tissue was fixed with formaldehyde and a heat mediated antigen retrival step was performed. Blocking of the sample was done with 5 minutes of peroxidase block and 10 minutes of protein block, followed by staining with ab39960 at 1/200 for 45 minutes at 20°C. A HRP conjugated goat anti-rabbit polyclonal antibody was used as the secondary antibody.

Anti-Endothelin B Receptor antibody (ab39960)参考文献

This product has been referenced in:
  • Hall SM  et al. Endothelin receptor expression in idiopathic pulmonary arterial hypertension: effect of bosentan and epoprostenol treatment. Eur Respir J 38:851-60 (2011). WB ; Human . Read more (PubMed: 21406517) »
  • Spinella F  et al. Endothelin-1 inhibits prolyl hydroxylase domain 2 to activate hypoxia-inducible factor-1alpha in melanoma cells. PLoS One 5:e11241 (2010). WB ; Human . Read more (PubMed: 20574527) »

See all 3 Publications for this product

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Human Tissue sections (Placenta)
Specification Placenta
Fixative Formaldehyde
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: Dako Target Retreival Solution
Permeabilization No
Blocking step 5 minutes of peroxidase block then 10 minutes of protein block. These are ready-to-use reagents purchased from Dako as blocking agent for 15 minute(s) · Concentration: 100% · Temperature: 20°C
Username

Mr. Antibody Solutions

Verified customer

提交于 Aug 22 2011

Application Immunocytochemistry/ Immunofluorescence
Sample Human Cell (HeLa)
Specification HeLa
Fixative Methanol
Permeabilization No
Username

Dr. Kirk McManus

Verified customer

提交于 May 09 2008

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"