Anti-Endothelin B Receptor抗体(ab117529)

概述

  • 产品名称
    Anti-Endothelin B Receptor抗体
    参阅全部 Endothelin B Receptor 一抗
  • 描述
    兔多克隆抗体to Endothelin B Receptor
  • 经测试应用
    适用于: ICC, IHC-Fr, WB, IHC-P, ICC/IFmore details
  • 种属反应性
    与反应: Mouse, Human
    预测可用于: Rabbit, Goat, Horse, Chicken, Cow, Dog, Pig, Rhesus monkey
  • 免疫原

    Synthetic peptide:

    KANDHGYDNFRSSNKYSSS

    conjugated to KLH, corresponding to C terminal amino acids 424-442 of Mouse Endothelin B Receptor.

  • 阳性对照
    • Mouse lung tissue; Mouse brain lysate; Endothelin B Receptor transfected cells

性能

  • 形式
    Liquid
  • 存放说明
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • 存储溶液
    Preservative: 0.02% Sodium azide
    Constituent: 99% Whole serum
  • 纯度
    Whole antiserum
  • 克隆
    多克隆
  • 同种型
    IgG
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab117529 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ICC 1/5000.
IHC-Fr 1/500 - 1/2000.
WB 1/5000. Predicted molecular weight: 50 kDa.
IHC-P 1/2000.
ICC/IF 1/5000.

靶标

  • 功能
    Non-specific receptor for endothelin 1, 2, and 3. Mediates its action by association with G proteins that activate a phosphatidylinositol-calcium second messenger system.
  • 组织特异性
    Expressed in placental stem villi vessels, but not in cultured placental villi smooth muscle cells.
  • 疾病相关
    Defects in EDNRB are a cause of Waardenburg syndrome type 4A (WS4A) [MIM:277580]; also known as Waardenburg-Shah syndrome. WS4A is characterized by the association of Waardenburg features (depigmentation and deafness) and the absence of enteric ganglia in the distal part of the intestine (Hirschsprung disease).
    Defects in EDNRB are the cause of Hirschsprung disease type 2 (HSCR2) [MIM:600155]; also known as aganglionic megacolon (MGC). HSCR2 is a congenital disorder characterized by absence of enteric ganglia along a variable length of the intestine. It is the most common cause of congenital intestinal obstruction. Early symptoms range from complete acute neonatal obstruction, characterized by vomiting, abdominal distention and failure to pass stool, to chronic constipation in the older child.
    Defects in EDNRB are the cause of ABCD syndrome (ABCDS) [MIM:600501]. ABCD syndrome is an autosomal recessive syndrome characterized by albinism, black lock at temporal occipital region, bilateral deafness, aganglionosis of the large intestine and total absence of neurocytes and nerve fibers in the small intestine.
  • 序列相似性
    Belongs to the G-protein coupled receptor 1 family. Endothelin receptor subfamily. EDNRB sub-subfamily.
  • 翻译后修饰
    Palmitoylation of Cys-402 was confirmed by the palmitoylation of Cys-402 in a deletion mutant lacking both Cys-403 and Cys-405.
  • 细胞定位
    Cell membrane.
  • Information by UniProt
  • 数据库链接
  • 别名
    • ABCDS antibody
    • Ednra antibody
    • EDNRB antibody
    • EDNRB_HUMAN antibody
    • Endothelin B receptor antibody
    • Endothelin B receptor precursor antibody
    • Endothelin receptor Non selective type antibody
    • Endothelin receptor non-selective type antibody
    • Endothelin receptor type B antibody
    • ET B antibody
    • ET-B antibody
    • ET-BR antibody
    • ETB antibody
    • ETBR antibody
    • ETRB antibody
    • Hirschsprung disease 2 antibody
    • HSCR antibody
    • HSCR2 antibody
    • OTTHUMP00000018534 antibody
    • OTTHUMP00000178736 antibody
    • WS4A antibody
    see all

Anti-Endothelin B Receptor antibody 图像

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of mouse lung tissue sections labeling Endothelin B Receptor with ab117529.

  • Immunohistochemistry (Frozen sections) analysis of mouse skin tissue sections labeling Endothelin B Receptor with ab117529 at 1/800 dilution. Tissues were fixed for 10 minutes, blocked in 3% fish gelatin for 10-20 minutes, and probed with an Isotype Control Antibody (left panel) (1/800 dilution), and Anti-Endothelin B Receptor antibody (ab117529) (right panel) overnight at 4ºC. Detection was performed using a PE-conjugated anti-rabbit IgG secondary antibody at a dilution of 1/800 (red). Nuclei were stained with DAPI and tissues were visualized by fluorescence microscopy.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of mouse lung tissue sections labeling Endothelin B Receptor with ab117529 at 1/1500 dilution. Tissue was fixed with formaldehyde; heat mediated antigen retrieval was performed using a citrate buffer pH 6.0. The tissue was blocked with 3% serum for 30 minutes at 20°C. The tissue was incubated with Anti-Endothelin B Receptor antibody (ab117529) for 12 hours in PBS at 20°C. A biotin conjugated goat anti-rabbit secondary antibody at 1/200 dilution.

    See Abreview

  • ab117529, at 1/5000 dilution, staining Endothelin B Receptor in HEK293 cells by Immunocytochemistry.
  • Anti-Endothelin B Receptor antibody (ab117529) at 1/5000 dilution + Mouse brain lysate

    Predicted band size : 50 kDa

Anti-Endothelin B Receptor antibody (ab117529)参考文献

This product has been referenced in:
  • Gulati S  et al. Attenuation of opioid tolerance by ETB receptor agonist, IRL-1620, is independent of an accompanied decrease in nerve growth factor in mice. Heliyon 3:e00317 (2017). WB ; Mouse . Read more (PubMed: 28626808) »
  • Sun D  et al. Hypercholesterolemia Impairs Nonstenotic Kidney Outcomes After Reversal of Experimental Renovascular Hypertension. Am J Hypertens 29:853-9 (2016). Read more (PubMed: 26739189) »

See all 3 Publications for this product

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Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (Blood)
Permeabilization
No
Specification
Blood
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 0.2% · Temperature: 37°C
Fixative
life cell staining
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Verified customer

提交于 Oct 13 2015

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Blocking step
Serum as blocking agent for 30 minute(s) · Concentration: 3% · Temperature: 20°C
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Citrat-Buffer pH 6,0
Sample
Mouse Tissue sections (lung)
Specification
lung
Permeabilization
No
Fixative
Formaldehyde
Username

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Verified customer

提交于 Jun 05 2013

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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