Anti-Endothelin A Receptor抗体(ab85163)

概述

  • 产品名称Anti-Endothelin A Receptor抗体
    参阅全部 Endothelin A Receptor 一抗
  • 描述
    兔多克隆抗体to Endothelin A Receptor
  • 经测试应用适用于: IHC-Fr, WBmore details
  • 种属反应性
    与反应: Rat
    预测可用于: Mouse, Rabbit, Dog, Human
  • 免疫原

    Synthetic peptide conjugated to KLH derived from within residues 100 - 200 of Rat Endothelin A Receptor.

    (Peptide available as ab93498.)

  • 阳性对照
    • This antibody gave a positive signal in the following rat tissue lysates: Brain; Cerebellum.

性能

应用

Our Abpromise guarantee covers the use of ab85163 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
IHC-Fr Use at an assay dependent concentration.
WB Use a concentration of 1 µg/ml. Detects a band of approximately 46 kDa (predicted molecular weight: 48 kDa).

靶标

  • 功能Receptor for endothelin-1. Mediates its action by association with G proteins that activate a phosphatidylinositol-calcium second messenger system. The rank order of binding affinities for ET-A is: ET1 > ET2 >> ET3.
  • 组织特异性Isoform 1, isoform 3 and isoform 4 are expressed in a variety of tissues, with highest levels in the aorta and cerebellum, followed by lung, atrium and cerebral cortex, lower levels in the placenta, kidney, adrenal gland, duodenum, colon, ventricle and liver but no expression in umbilical vein endothelial cells. Within the placenta, isoform 1, isoform 2, isoform 3 and isoform 4 are expressed in the villi and stem villi vessels.
  • 序列相似性Belongs to the G-protein coupled receptor 1 family. Endothelin receptor subfamily. EDNRA sub-subfamily.
  • 细胞定位Cell membrane.
  • Information by UniProt
  • 数据库链接
  • 别名
    • Ednra antibody
    • EDNRA_HUMAN antibody
    • Endothelin 1 receptor precursor antibody
    • Endothelin 1 specific receptor antibody
    • Endothelin A receptor antibody
    • Endothelin ET A receptor antibody
    • Endothelin receptor ET1 specific type antibody
    • Endothelin receptor subtype A antibody
    • Endothelin receptor type A antibody
    • Endothelin-1 receptor antibody
    • ET A antibody
    • ET-A antibody
    • ETA antibody
    • ETA R antibody
    • ETA-R antibody
    • ETAR antibody
    • ETRA antibody
    • hET AR antibody
    • hET-AR antibody
    see all

Anti-Endothelin A Receptor antibody 图像

  • All lanes : Anti-Endothelin A Receptor antibody (ab85163) at 1 µg/ml

    Lane 1 : Brain (Rat) Tissue Lysate
    Lane 2 : Cerebellum Rat Tissue Lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 48 kDa
    Observed band size : 46 kDa (why is the actual band size different from the predicted?)


    Exposure time : 20 minutes
    The band observed at 46 kDa could potentially be a cleaved form of Rat Endothelin-1 receptor precursor, as the target protein contains a 20 amino acid signal peptide, which when removed gives an expected molecular weight of 46 kDa.
  • All lanes : Anti-Endothelin A Receptor antibody (ab85163) at 1/250 dilution (in 5% BSA + TBS + 0.1% Tween20 for 12 hours at 4°C)

    Lane 1 : Whole tissue lysate of rat lung
    Lane 2 : Whole tissue lysate of rat lung

    Lysates/proteins at 40 µg per lane.

    Secondary
    An HRP-conjugated Goat anti-rabbit IgG polyclonal at 1/1000 dilution
    Developed using the ECL technique

    Performed under non-reducing conditions.

    Predicted band size : 48 kDa
    Observed band size : 48 kDa


    Exposure time : 30 seconds

    This image is courtesy of an Abreview submitted by Nico Jentsch

    Blocking step: 5% Milk for 1 hour at 23°C

    See Abreview

Anti-Endothelin A Receptor antibody (ab85163)参考文献

This product has been referenced in:
  • Joseph EK  et al. Vascular endothelial cells mediate mechanical stimulation-induced enhancement of endothelin hyperalgesia via activation of P2X2/3 receptors on nociceptors. J Neurosci 33:2849-59 (2013). WB ; Rat . Read more (PubMed: 23407944) »
  • Speed JS  et al. Renal medullary endothelin-1 is decreased in Dahl salt-sensitive rats. Am J Physiol Regul Integr Comp Physiol 301:R519-23 (2011). WB ; Rat . Read more (PubMed: 21613578) »

See all 2 Publications for this product

Product Wall

Application Immunocytochemistry/ Immunofluorescence
Sample Human Cell (Blood)
Permeabilization No
Specification Blood
Blocking step Serum as blocking agent for 30 minute(s) · Concentration: 0.2% · Temperature: 4°C
Fixative Paraformaldehyde
Username

Abcam user community

Verified customer

提交于 Oct 13 2015

Thank you for your follow up email and I am sorry that I did not notice it until I had already sent your reply.

You are correct in thinking that the anti-Endothelin A Receptor antibody (ab85163) has been used successfully in staining frozen t...

Read More
Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunohistochemistry (Frozen sections)
Sample Rat Tissue sections (lung tissue)
Specification lung tissue
Fixative Acetone
Permeabilization No
Blocking step SuperBlock Blocking Buffer as blocking agent for 20 minute(s) · Concentration: 100% · Temperature: 23°C
Username

Abcam user community

Verified customer

提交于 Dec 02 2010

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Rat Tissue lysate - whole (rat (lung))
Loading amount 40 µg
Specification rat (lung)
Gel Running Conditions Non-reduced Denaturing
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 23°C
Username

Mr. Nico Jentsch

Verified customer

提交于 Jun 22 2010

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"