Anti-Endothelin A Receptor抗体(ab84673)

概述

  • 产品名称Anti-Endothelin A Receptor抗体
    参阅全部 Endothelin A Receptor 一抗
  • 描述
    兔多克隆抗体to Endothelin A Receptor
  • 经测试应用适用于: WB, ICC/IFmore details
  • 种属反应性
    与反应: Human
    预测可用于: Rabbit, Dog
  • 免疫原

    Synthetic peptide conjugated to KLH derived from within residues 50 - 150 of Human Endothelin A Receptor.

  • 阳性对照
    • This antibody gave a positive signal in the following whole cell lysates: HeLa; HepG2; MCF7

性能

相关产品

应用

Our Abpromise guarantee covers the use of ab84673 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use a concentration of 1 µg/ml. Detects a band of approximately 49 kDa (predicted molecular weight: 49 kDa).
ICC/IF Use a concentration of 1 µg/ml.

靶标

  • 功能Receptor for endothelin-1. Mediates its action by association with G proteins that activate a phosphatidylinositol-calcium second messenger system. The rank order of binding affinities for ET-A is: ET1 > ET2 >> ET3.
  • 组织特异性Isoform 1, isoform 3 and isoform 4 are expressed in a variety of tissues, with highest levels in the aorta and cerebellum, followed by lung, atrium and cerebral cortex, lower levels in the placenta, kidney, adrenal gland, duodenum, colon, ventricle and liver but no expression in umbilical vein endothelial cells. Within the placenta, isoform 1, isoform 2, isoform 3 and isoform 4 are expressed in the villi and stem villi vessels.
  • 序列相似性Belongs to the G-protein coupled receptor 1 family. Endothelin receptor subfamily. EDNRA sub-subfamily.
  • 细胞定位Cell membrane.
  • Information by UniProt
  • 数据库链接
  • 别名
    • Ednra antibody
    • EDNRA_HUMAN antibody
    • Endothelin 1 receptor precursor antibody
    • Endothelin 1 specific receptor antibody
    • Endothelin A receptor antibody
    • Endothelin ET A receptor antibody
    • Endothelin receptor ET1 specific type antibody
    • Endothelin receptor subtype A antibody
    • Endothelin receptor type A antibody
    • Endothelin-1 receptor antibody
    • ET A antibody
    • ET-A antibody
    • ETA antibody
    • ETA R antibody
    • ETA-R antibody
    • ETAR antibody
    • ETRA antibody
    • hET AR antibody
    • hET-AR antibody
    see all

Anti-Endothelin A Receptor antibody 图像

  • All lanes : Anti-Endothelin A Receptor antibody (ab84673) at 1 µg/ml

    Lane 1 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
    Lane 2 : HepG2 (Human hepatocellular liver carcinoma cell line) Whole Cell Lysate
    Lane 3 : MCF7 (Human breast adenocarcinoma cell line) Whole Cell Lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (HRP), pre-adsorbed at 1/5000 dilution

    Predicted band size : 49 kDa
    Observed band size : 49 kDa


    Exposure time : 4 minutes
  • ICC/IF image of ab84673 stained HeLa cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab84673, 5µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899 Dylight 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

Anti-Endothelin A Receptor antibody (ab84673)参考文献

ab84673 has not yet been referenced specifically in any publications.

Product Wall

Thank you for contacting Abcam regarding our anti-Endothelin Receptor A antibodies. Regarding these antibodies, all of the information that is currently available can be found on the product datasheets on our website. I have summarized the answers to y...

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Thank you for your inquiry. The recipe for RIPA buffer is below: RIPA buffer (radio immuno precipitation assay) buffer: RIPA buffer contains the ionic detergent sodium deoxycholate as an active constituent and is particularly used for nuclear membr...

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