Anti-Endothelin 1抗体(ab117757)

概述

  • 产品名称Anti-Endothelin 1抗体
    参阅全部 Endothelin 1 一抗
  • 描述
    兔多克隆抗体to Endothelin 1
  • 经测试应用适用于: WB, IHC-P, ICCmore details
  • 种属反应性
    与反应: Mouse, Rat, Human
  • 免疫原

    Synthetic peptide conjugated to KLH, corresponding to CS-Abu-SSLMDKE-Abu-VYF-Abu-HLDIIW of Mouse Endothelin 1.

  • 阳性对照
    • Mouse dorsal root ganglia tissue.

性能

应用

Our Abpromise guarantee covers the use of ab117757 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/5000. Predicted molecular weight: 23 kDa.
IHC-P 1/2000.
ICC 1/5000.

靶标

Anti-Endothelin 1 antibody 图像

  • ab117757 at 1/2000 dilution staining Endothelin 1 in Mouse Dorsal root ganglia by Immunohistochemistry.

Anti-Endothelin 1 antibody (ab117757)参考文献

ab117757 has not yet been referenced specifically in any publications.

Product Wall

Thank you for contacting us and your interest in our products.

I am sorry for the delay in getting back to you. I can confirm that the Anti-Endothelin 1 antibody (ab117757) has been used to perform IHC staining with paraffin embedded tissue wi...

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Wenn wir keine genaueren Angaben zum Protokoll machen (wie in diesem Fall), bedeutet dies, dass Sie eigentlich ohne Probleme Ihr Standard-Protokoll verwenden können (Optimierungsschritte eingeschlossen).


Unfortunately, we do not have the blocking peptide for ab117757 available for sale. I apologize for saying it would be likely that we can make it available. If you cannot find an alternative blocking peptide source that overlaps the immunogen sequ...

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Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Rat Tissue lysate - whole (liver)
Loading amount 30 µg
Specification liver
Treatment 2mg/kg LPS 6h
Gel Running Conditions Reduced Denaturing
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
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Verified customer

提交于 Nov 20 2012

Thank you for your inquiry and I am sorry to hear that you are having trouble with this antibody. I've copied our protocol below. Please letme know if this is similar to what you've tried.


Western Blot Protocol


Polyacryl...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"