Anti-Endothelial Cell抗体[RECA-1] (ab9774)

概述

  • 产品名称
    Anti-Endothelial Cell抗体[RECA-1]
    参阅全部 Endothelial Cell 一抗
  • 描述
    小鼠单克隆抗体[RECA-1] to Endothelial Cell
  • 经测试应用
    适用于: ICC/IF, IHC-P, IHC-G, IHC-Fr, IHC-FrFlmore details
  • 种属反应性
    与反应: Rat
  • 免疫原

    Peripheral and mesenteric lymph nodes from AO rat

  • 阳性对照
    • Rat brain FFPE section (IHC)
  • 常规说明

    This antibody clone is manufactured by Abcam.

    This antibody is available as an azide-free product (see ab172579).

    RECA-1 reacts with rat endothelial cell antigen and showed to be reactive with all vascular endothelium in three tested rat strains of different MHC-haplotype; Lewis (TR-11), BN (RT-1n) and OA (RT-1u). RECA-1 recognises a cell surface antigen. The antibody has been successfully applied in staining of viable endothelial cells in vitro, and of vascular endothelium in vivo. No reactivity of RECA-1 was seen with other cells e.g. fibroblasts, leukocytes and non endothelial stromal cells nor with other various tested species other than rat e.g. mouse, rabbit, sheep, goat and human. RECA-1 is a promising antibody for rat endothelial cell studies, and in particular for further defining nature and function of endothelial cell-specific antigens.

性能

  • 形式
    Liquid
  • 存放说明
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term.
  • 存储溶液
    pH: 7.40
    Preservative: 0.02% Sodium azide
    Constituent: PBS

    Please note that some batches of ab9774 may contain 0.4M arginine. Please contact Scientific Support for further information.
  • Concentration information loading...
  • 纯度
    Immunogen affinity purified
  • Primary antibody说明
    RECA-1 reacts with rat endothelial cell antigen and showed to be reactive with all vascular endothelium in three tested rat strains of different MHC-haplotype; Lewis (TR-11), BN (RT-1n) and OA (RT-1u). RECA-1 recognises a cell surface antigen. The antibody has been successfully applied in staining of viable endothelial cells in vitro, and of vascular endothelium in vivo. No reactivity of RECA-1 was seen with other cells e.g. fibroblasts, leukocytes and non endothelial stromal cells nor with other various tested species other than rat e.g. mouse, rabbit, sheep, goat and human. RECA-1 is a promising antibody for rat endothelial cell studies, and in particular for further defining nature and function of endothelial cell-specific antigens.
  • 克隆
    单克隆
  • 克隆编号
    RECA-1
  • 同种型
    IgG1
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab9774 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ICC/IF Use at an assay dependent concentration. Fix tissue in 2% paraformaldehyde in PBS (pH 7.4) for 3 hours and maintain overnight in 6% sucrose in PBS, rinse with acetone, and embed in Technovit 8100 (glycol methacrylate). Do not perform antigen retrieval (see Duijvestejn AM et al. for details).
IHC-P Use a concentration of 10 µg/ml.
IHC-G 1/10 - 1/50.
IHC-Fr 1/50.
IHC-FrFl Use at an assay dependent concentration.

靶标

  • 相关性
    The endothelium is a single layer of cells lining various organs and cavities of the body, especially the blood vessels, heart, and lymphatic vessels.Endothelial cells line the entire circulatory system, from the heart to the smallest capillary. Endothelial cells are involved in many aspects of vascular biology, such as vasoconstriction and vasodilation, and hence the control of blood pressure; blood clotting (thrombosis & fibrinolysis); atherosclerosis; formation of new blood vessels (angiogenesis) and inflammation and swelling (oedema). Endothelial cells also control the passage of materials, and the transit of white blood cells, into and out of the bloodstream.

Anti-Endothelial Cell antibody [RECA-1] 图像

  • IHC image of Endothelial Cell staining in Rat brain FFPE section, performed on a BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab9774, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX
  • ab9774 staining cells of rat liver sections by IHC-Fr.  Cells were acetone fixed and blocked with 1% BSA for 5 minutes at 25°C prior to incubating with ab9774 diluted 1/25 for 30 minutes at 25°C.  An Alexa Fluor® 488 conjugated goat anti-mouse antibody was used as the secondary.

    Double IIF labeling with RECA-1 (Green) and dipeptidyl peptidase IV (Red)

    See Abreview

  • ab9774 staining endothelial cells in Rat lung tissue by Immunohistochemistry (Frozen sections). The sections were fixed in acetone prior to blocking with 100% SuperBlock Blocking Buffer for 20 minutess at 23°C. The primary antibody was diluted 1/100 and incubated with the sample for 12 hours at 4°C. A Biotin-conjugated Rabbit anti-Mouse polyclonal was used as the secondary antibody, diluted 1/200.

    See Abreview

Anti-Endothelial Cell antibody [RECA-1] (ab9774)参考文献

This product has been referenced in:
  • Kimura-Ohba S  et al. Transient increase of fractional anisotropy in reversible vasogenic edema. J Cereb Blood Flow Metab 36:1731-1743 (2016). Read more (PubMed: 26865662) »
  • Miyanohara A  et al. Potent spinal parenchymal AAV9-mediated gene delivery by subpial injection in adult rats and pigs. Mol Ther Methods Clin Dev 3:16046 (2016). IHC-FoFr . Read more (PubMed: 27462649) »

See all 29 Publications for this product

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunohistochemistry free floating
Sample
Rat Tissue sections (Rat striatum, endothelial cells)
Specification
Rat striatum, endothelial cells
Username

Dr. Edward Fletcher

Verified customer

提交于 Jul 13 2017

Application
Immunohistochemistry (PFA perfusion fixed frozen sections)
Sample
Rat Tissue sections (Brain)
Antigen retrieval step
Other
Permeabilization
Yes - triton 1%
Specification
Brain
Blocking step
Serum as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
Fixative
Formaldehyde
Username

Mrs. Francoise Geffroy

Verified customer

提交于 Jan 11 2017

Application
Immunohistochemistry (Frozen sections)
Sample
Rat Tissue sections (Liver)
Permeabilization
No
Specification
Liver
Blocking step
Serum as blocking agent for 30 minute(s) · Concentration: 1.5% · Temperature: 20°C
Fixative
Acetone
Username

Dr. Fabio Marongiu

Verified customer

提交于 Oct 20 2015

Application
Immunohistochemistry (PFA perfusion fixed frozen sections)
Sample
Rat Tissue sections (Brain tissue)
Antigen retrieval step
None
Specification
Brain tissue
Blocking step
Serum as blocking agent for 20 minute(s) · Concentration: 10 · Temperature: 24°C
Fixative
Formaldehyde
Username

Sumit Sarkar

Verified customer

提交于 Aug 14 2015

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Blocking step
BSA as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 24°C
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: sodium citrate pH=6
Sample
Rat Tissue sections (omentum)
Specification
omentum
Permeabilization
No
Fixative
Formaldehyde
Username

Abcam user community

Verified customer

提交于 Jan 09 2014

Unfortunately this clone, RECA-1, for anti-Anti-Endothelial Cell antibody is produced only in mouse. I have reviewed other products on our catalog and it appears that none of our other Anti-Endothelial Cell antibodies are produced in rabbit either. I a...

Read More
Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Rat Tissue sections (Heart)
Specification
Heart
Fixative
10% Neutral Buffered Formalin, 24h
Antigen retrieval step
Enzymatic
Permeabilization
No
Username

Abcam user community

Verified customer

提交于 Oct 23 2012

Thank you for contacting us.

ab9774 is a well characterized antibody is fully guaranteed to detect the endothelial cells. Please use the product without any hesitation.

ab22492 is also reactive against vascular endothelium so this pro...

Read More
Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Rat Tissue sections (Kidneys)
Specification
Kidneys
Fixative
Formaldehyde
Antigen retrieval step
Enzymatic - Buffer/Enzyme Used: Proteinase K
Permeabilization
No
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 25°C
Username

Abcam user community

Verified customer

提交于 Jun 30 2012

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunohistochemistry (Frozen sections)
Sample
Rat Tissue sections (Kidneys)
Specification
Kidneys
Fixative
Formaldehyde
Permeabilization
No
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 25°C
Username

Abcam user community

Verified customer

提交于 Jun 30 2012

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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