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Peripheral and mesenteric lymph nodes from AO rat
This antibody is available as an azide-free product (see ab172579).
RECA-1 reacts with rat endothelial cell antigen and showed to be reactive with all vascular endothelium in three tested rat strains of different MHC-haplotype; Lewis (TR-11), BN (RT-1n) and OA (RT-1u). RECA-1 recognises a cell surface antigen. The antibody has been successfully applied in staining of viable endothelial cells in vitro, and of vascular endothelium in vivo. No reactivity of RECA-1 was seen with other cells e.g. fibroblasts, leukocytes and non endothelial stromal cells nor with other various tested species other than rat e.g. mouse, rabbit, sheep, goat and human. RECA-1 is a promising antibody for rat endothelial cell studies, and in particular for further defining nature and function of endothelial cell-specific antigens.
Our Abpromise guarantee covers the use of ab9774 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ICC/IF||Use at an assay dependent concentration. Fix tissue in 2% paraformaldehyde in PBS (pH 7.4) for 3 hours and maintain overnight in 6% sucrose in PBS, rinse with acetone, and embed in Technovit 8100 (glycol methacrylate). Do not perform antigen retrieval (see Duijvestejn AM et al. for details).|
|IHC-P||Use a concentration of 10 µg/ml.|
|IHC-G||1/10 - 1/50.|
|IHC-FrFl||Use at an assay dependent concentration.|
ab9774 staining cells of rat liver sections by IHC-Fr. Cells were acetone fixed and blocked with 1% BSA for 5 minutes at 25°C prior to incubating with ab9774 diluted 1/25 for 30 minutes at 25°C. An Alexa Fluor® 488 conjugated goat anti-mouse antibody was used as the secondary.
Double IIF labeling with RECA-1 (Green) and dipeptidyl peptidase IV (Red)
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"