Anti-Endostatin抗体(ab3453)

概述

  • 产品名称Anti-Endostatin抗体
    参阅全部 Endostatin 一抗
  • 描述
    兔多克隆抗体to Endostatin
  • 特异性Detects recombinant human endostatin.
  • 经测试应用适用于: ICC, ICC/IF, IHC-Pmore details
  • 种属反应性
    与反应: Mouse, Human, Non Human Primates
  • 免疫原

    Synthetic peptide corresponding to Human Endostatin aa 129-142.
    Sequence:

    RRLM/TESYCETWRTE


    (Peptide available as ab4983)

  • 常规说明

    ab3453 has been recombinant only tested in Western blot

性能

相关产品

应用

Our Abpromise guarantee covers the use of ab3453 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ICC Use at an assay dependent concentration.
ICC/IF 1/200 - 1/1000.
IHC-P 1/100 - 1/500.

靶标

  • 功能COLA18A probably plays a major role in determining the retinal structure as well as in the closure of the neural tube.
    Endostatin potently inhibits endothelial cell proliferation and angiogenesis. May inhibit angiogenesis by binding to the heparan sulfate proteoglycans involved in growth factor signaling.
  • 组织特异性Present in multiple organs with highest levels in liver, lung and kidney.
  • 疾病相关Defects in COL18A1 are a cause of Knobloch syndrome (KNO) [MIM:267750]. KNO is an autosomal recessive disorder defined by the occurrence of high myopia, vitreoretinal degeneration with retinal detachment, macular abnormalities and occipital encephalocele.
  • 序列相似性Belongs to the multiplexin collagen family.
    Contains 1 FZ (frizzled) domain.
    Contains 1 TSP N-terminal (TSPN) domain.
  • 翻译后修饰Prolines at the third position of the tripeptide repeating unit (G-X-Y) are hydroxylated in some or all of the chains.
  • 细胞定位Secreted > extracellular space > extracellular matrix.
  • Information by UniProt
  • 数据库链接
  • 别名
    • Alpha 1 collagen type 18 (XVIII)(COL18A1) antibody
    • Alpha 1 type XVIII collagen antibody
    • Antiangiogenic agent antibody
    • COIA1_HUMAN antibody
    • COL15A1 antibody
    • Col18a1 antibody
    • Collagen alpha 1(XV) chain antibody
    • Collagen alpha 1(XVIII) chain antibody
    • Collagen alpha-1(XV) chain antibody
    • Collagen type XV proteoglycan antibody
    • Collagen type XVIII alpha 1 antibody
    • Collagen XV, alpha 1 polypeptide antibody
    • Collagen, type XV, alpha 1 antibody
    • Endostatin antibody
    • Endostatin XV antibody
    • FLJ27325 antibody
    • FLJ34914 antibody
    • FLJ38566 antibody
    • KNO antibody
    • KNO1 antibody
    • KS antibody
    • MGC74745 antibody
    • Multi functional protein MFP antibody
    • OTTHUMP00000021782 antibody
    • OTTHUMP00000115472 antibody
    • OTTHUMP00000115473 antibody
    see all

Anti-Endostatin antibody 图像

  • ab3453 labelling Endostatin (green) in 293T cells (right) compared with a negative control (left) by Immunocytochemistry/Immunofluorscence. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes, blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were incubated with the primary antibody (1:200 in 3% BSA-PBS) overnight at 4 ºC. A DyLight 488-conjugated Goat anti-rabbit IgG was used as the secondary antibody. Red (phalloidin) - F-actin, Blue (DAPI) - nuclei (blue). Images were taken at a magnification of 60x.

  • ab3453 labelling Endostatin (green) in A431 cells (right) compared with a negative control (left) by Immunocytochemistry/Immunofluorscence. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes, blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were incubated with the primary antibody (1:200 in 3% BSA-PBS) overnight at 4 ºC. A DyLight 488-conjugated Goat anti-rabbit IgG was used as the secondary antibody. Red (phalloidin) - F-actin, Blue (DAPI) - nuclei (blue). Images were taken at a magnification of 60x.

  • ab3453 labelling Endostatin (green) in NIH-3T3 cells (right) compared with a negative control (left) by Immunocytochemistry/Immunofluorscence. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes, blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were incubated with the primary antibody (1:200 in 3% BSA-PBS) overnight at 4 ºC. A DyLight 488-conjugated Goat anti-rabbit IgG was used as the secondary antibody. Red (phalloidin) - F-actin, Blue (DAPI) - nuclei (blue). Images were taken at a magnification of 60x.

  • ab3453 labelling Endostatin in the secretion of Human kidney tissue (right) compared with a negative control (left) by Immunohistochemistry (formalin/PFA-fixed paraffin embedded sections). To expose target proteins, antigen retrieval method was performed using 10mM sodium citrate (pH 6.0), microwaved for 8-15 min. Following antigen retrieval, tissues were blocked in 3% H2O2-methanol for 15 min at room temperature. Tissue sections were incuabted with the primary antibody (1:100 in 3% BSA-PBS) overnight at 4°C. A HRP-conjugated anti-rabbit IgG was used as the secondary antibody, followed by colorimetric detection using a DAB kit. Tissues were counterstained with hematoxylin and dehydrated with ethanol and xylene to prep for mounting.

  • ab3453 labelling Endostatin in the secretion of Human colon tissue (right) compared with a negative control (left) by Immunohistochemistry (formalin/PFA-fixed paraffin embedded sections). To expose target proteins, antigen retrieval method was performed using 10mM sodium citrate (pH 6.0), microwaved for 8-15 min. Following antigen retrieval, tissues were blocked in 3% H2O2-methanol for 15 min at room temperature. Tissue sections were incuabted with the primary antibody (1:200 in 3% BSA-PBS) overnight at 4°C. A HRP-conjugated anti-rabbit IgG was used as the secondary antibody, followed by colorimetric detection using a DAB kit. Tissues were counterstained with hematoxylin and dehydrated with ethanol and xylene to prep for mounting.

  • ab3453 labelling Endostatin in the secretion of Mouse kidney tissue (right) compared with a negative control (left) by Immunohistochemistry (formalin/PFA-fixed paraffin embedded sections). To expose target proteins, antigen retrieval method was performed using 10mM sodium citrate (pH 6.0), microwaved for 8-15 min. Following antigen retrieval, tissues were blocked in 3% H2O2-methanol for 15 min at room temperature. Tissue sections were incuabted with the primary antibody (1:100 in 3% BSA-PBS) overnight at 4°C. A HRP-conjugated anti-rabbit IgG was used as the secondary antibody, followed by colorimetric detection using a DAB kit. Tissues were counterstained with hematoxylin and dehydrated with ethanol and xylene to prep for mounting.

Anti-Endostatin antibody (ab3453)参考文献

ab3453 has not yet been referenced specifically in any publications.

Product Wall

Thank you for your reply.


Yes, my apologies, the µ symbol did not paste correctly from the protocol. 100 ug were resolved on 10% SDS gels under reducing conditions.


The recommended working dilution of the antibody ...

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Thank you for contacting us. I am sorry to hear that this antibody is not providing satisfactory results.

The lab had tested the below protocol:

Tumours injected 2 days earlier with angiostatin or empty expression plasmids were exc...

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