The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
应用说明ELISA: Use at a concentration of 0.5 - 1 µg/ml. The detection limit for recombinant mouse endostatin is approximately 6 ng/well.
WB: Use at a concentration of 1 - 2 µg/ml. The detection limit for recombinant mouse endostatin is approximately 50 ng/lane and 5 ng/lane under non-reducing and reducing conditions, respectively. Predicted molecular weight: 22 kDa.
Not tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
功能COLA18A probably plays a major role in determining the retinal structure as well as in the closure of the neural tube. Endostatin potently inhibits endothelial cell proliferation and angiogenesis. May inhibit angiogenesis by binding to the heparan sulfate proteoglycans involved in growth factor signaling.
组织特异性Present in multiple organs with highest levels in liver, lung and kidney.
疾病相关Defects in COL18A1 are a cause of Knobloch syndrome (KNO) [MIM:267750]. KNO is an autosomal recessive disorder defined by the occurrence of high myopia, vitreoretinal degeneration with retinal detachment, macular abnormalities and occipital encephalocele.
序列相似性Belongs to the multiplexin collagen family. Contains 1 FZ (frizzled) domain. Contains 1 TSP N-terminal (TSPN) domain.
翻译后修饰Prolines at the third position of the tripeptide repeating unit (G-X-Y) are hydroxylated in some or all of the chains.
细胞定位Secreted > extracellular space > extracellular matrix.