Anti-Endomucin抗体[V.7C7.1] (ab106100)

概述

  • 产品名称Anti-Endomucin抗体[V.7C7.1]
    参阅全部 Endomucin 一抗
  • 描述
    大鼠单克隆抗体[V.7C7.1] to Endomucin
  • 经测试应用适用于: WB, IP, IHC-P, IHC-Fr, Flow Cytmore details
    不适用于: FuncS
  • 种属反应性
    与反应: Mouse
    不与反应: Human
  • 免疫原

    Mouse Endomucin-IgG fusion protein

性能

应用

Our Abpromise guarantee covers the use of ab106100 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/50. Predicted molecular weight: 27 kDa.
IP Use at an assay dependent concentration.
IHC-P Use at an assay dependent concentration.
IHC-Fr Use at an assay dependent concentration.
Flow Cyt 1/50. ab18450-Rat monoclonal IgG2a, is suitable for use as an isotype control with this antibody.
  • 应用说明Is unsuitable for FuncS.
  • 靶标

    • 功能Endothelial sialomucin, also called endomucin or mucin-like sialoglycoprotein, which interferes with the assembly of focal adhesion complexes and inhibits interaction between cells and the extracellular matrix.
    • 组织特异性Expressed in heart, kidney and lung.
    • 翻译后修饰Highly O-glycosylated. Sialic acid-rich glycoprotein.
    • 细胞定位Secreted and Cell membrane. Membrane. Consistently localized to the plasma membrane and less abundantly to Golgi-like perinuclear stacks.
    • Information by UniProt
    • 数据库链接
    • 别名
      • EMCN antibody
      • EMCN2 antibody
      • Endomucin 2 antibody
      • Endomucin antibody
      • Endomucin-2 antibody
      • Endothelial sialomucin antibody
      • Gastric cancer antigen Ga34 antibody
      • MUC-14 antibody
      • MUC14 antibody
      • MUCEN_HUMAN antibody
      • Mucin 14 antibody
      • Mucin-14 antibody
      see all

    Anti-Endomucin antibody [V.7C7.1] 图像

    • Immunohistochemical whole flat mounted mouse retinal tissue image. Primary antibody was used at a concentration of 1/100 and incubated at 4°C for 14 hours. A secondary donkey anti-rat Alexa Fluor® 488 preadsorbed antibody similar to ab150153 was used at a 1/400 concentration.

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    • ab106100 staining Endomucin in Mouse kidney tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with paraformaldehyde and blocked with 5% BSA / 5% Donkey Serum for an hour; antigen retrieval was by heat mediation in a Tris EDTA pH 9. Samples were incubated with primary antibody 1/100 for 14 hours at 4°C. An Alexa Fluor® 568 - conjugated Donkey anti-rat IgG polyclonal (1/400) was used as the secondary antibody.

      See Abreview

    Anti-Endomucin antibody [V.7C7.1] (ab106100)参考文献

    This product has been referenced in:
    • Shi X  et al. The transcription factor Mesp1 interacts with cAMP-responsive element binding protein 1 (Creb1) and coactivates Ets variant 2 (Etv2) gene expression. J Biol Chem 290:9614-25 (2015). Read more (PubMed: 25694434) »
    • Kuhn A  et al. Expression of endomucin, a novel endothelial sialomucin, in normal and diseased human skin. J Invest Dermatol 119:1388-93 (2002). Read more (PubMed: 12485444) »

    See all 3 Publications for this product

    Product Wall

    Application IHC - Wholemount
    Sample Mouse Tissue (Retina flat mount)
    Specification Retina flat mount
    Username

    Abcam user community

    Verified customer

    提交于 Aug 07 2015

    Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample Mouse Tissue sections (Kidney)
    Antigen retrieval step Heat mediated - Buffer/Enzyme Used: Tris EDTA pH 9
    Permeabilization Yes - 0.5% Triton X-100
    Specification Kidney
    Blocking step 5% BSA / 5% Donkey Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5%
    Fixative Paraformaldehyde
    Username

    Abcam user community

    Verified customer

    提交于 Aug 07 2015

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"