概述

  • 产品名称Anti-Endo G抗体
    参阅全部 Endo G 一抗
  • 描述
    兔多克隆抗体to Endo G
  • 经测试应用适用于: WBmore details
  • 种属反应性
    与反应: Mouse, Rat, Human
  • 免疫原

    Synthetic peptide conjugated to KLH derived from within residues 50 - 150 of Human Endo G.

    (Peptide available as ab66560.)

  • 阳性对照
    • This antibody gave a positive signal in the following lysates: Human Heart Tissue Mouse Spinal Cord Tissue Mouse Skeletal Muscle Tissue Rat Brain Tissue HeLa Whole Cell

性能

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应用

Our Abpromise guarantee covers the use of ab66561 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use a concentration of 1 µg/ml. Detects a band of approximately 40 kDa (predicted molecular weight: 35 kDa).Can be blocked with Human Endo G peptide (ab9648).

靶标

Anti-Endo G antibody 图像

  • All lanes : Anti-Endo G antibody (ab66561) at 1 µg/ml

    Lane 1 : Human heart tissue lysate - total protein (ab29431)
    Lane 2 : Spinal Cord (Mouse) Tissue Lysate
    Lane 3 : Rat Kidney Tissue Lysate
    Lane 4 : Mouse skeletal muscle tissue lysate - total protein (ab29711)
    Lane 5 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
    Lane 6 : Brain (Rat) Tissue Lysate - normal tissue

    Lysates/proteins at 10 µg per lane.

    Secondary
    Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution

    Performed under reducing conditions.

    Predicted band size : 35 kDa
    Observed band size : 40 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 60 kDa. We are unsure as to the identity of these extra bands.

Anti-Endo G antibody (ab66561)参考文献

ab66561 has not yet been referenced specifically in any publications.

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