概述

  • 产品名称Anti-ENAH抗体
    参阅全部 ENAH 一抗
  • 描述
    兔多克隆抗体to ENAH
  • 特异性Detects endogenous levels of total ENAH protein.
  • 经测试应用适用于: WB, ELISA, IHC-Pmore details
  • 种属反应性
    与反应: Mouse, Human
    预测可用于: Rat
  • 免疫原

    Synthesized peptide derived from internal of human ENAH.

  • 阳性对照
    • Extracts from HepG2 cells and human breast carcinoma tissue

性能

应用

Our Abpromise guarantee covers the use of ab92849 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB
ELISA
IHC-P
  • 应用说明ELISA: 1/10,000.
    IHC-P: 1/50 - 1/100.
    WB: 1/500 - 1/1000. Predicted molecular weight: 67 kDa.

    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • 靶标

    • 功能Ena/VASP proteins are actin-associated proteins involved in a range of processes dependent on cytoskeleton remodeling and cell polarity such as axon guidance and lamellipodial and filopodial dynamics in migrating cells. ENAH induces the formation of F-actin rich outgrowths in fibroblasts. Acts synergistically with BAIAP2-alpha and downstream of NTN1 to promote filipodia formation. Required for the actin-based mobility of Listeria monocytogenes.
    • 组织特异性Expressed in myoepithelia of parotid, breast, bronchial glands and sweat glands. Expressed in colon-rectum muscolaris mucosae epithelium, pancreas acinar ductal epithelium, endometrium epithelium, prostate fibromuscolar stroma and placenta vascular media. Overexpressed in a majority of breast cancer cell lines and primary breast tumor lesions.
    • 序列相似性Belongs to the Ena/VASP family.
      Contains 1 WH1 domain.
    • 结构域The EVH2 domain is comprised of 3 regions. Block A is a thymosin-like domain required for G-actin binding. The KLKR motif within this block is essential for the G-actin binding and for actin polymerization. Block B is required for F-actin binding and subcellular location, and Block C for tetramerization.
    • 翻译后修饰NTN1-induced PKA phosphorylation on Ser-265 directly parallels the formation of filopodial protrusions.
      Phosphorylated upon DNA damage, probably by ATM or ATR.
    • 细胞定位Cytoplasm. Cytoplasm > cytoskeleton. Cell projection > lamellipodium. Cell projection > filopodium. Cell junction > synapse. Cell junction > focal adhesion. Targeted to the leading edge of lamellipodia and filopodia by MRL family members. Colocalizes at filopodial tips with a number of other proteins including vinculin and zyxlin. Colocalizes with N-WASP at the leading edge. Colocalizes with GPHN and PFN at synapses.
    • Information by UniProt
    • 数据库链接
    • 别名
      • ENA antibody
      • Enah antibody
      • ENAH_HUMAN antibody
      • MENA antibody
      • NDPP1 antibody
      • Protein enabled homolog antibody
      see all

    Anti-ENAH antibody 图像

    • All lanes : Anti-ENAH antibody (ab92849) at 1/500 dilution

      Lane 1 : Extracts from HepG2 cells
      Lane 2 : Extracts from HepG2 cells with peptide at 10 µg

      Lysates/proteins at 30 µg per lane.


      Predicted band size : 67 kDa
    • Immunohistochemistry analysis of paraffin-embedded human breast carcinoma tissue, using ab92849 at 1/50-1/100 dilution with (+) and without (-) immunising peptide.

    Anti-ENAH antibody (ab92849)参考文献

    ab92849 has not yet been referenced specifically in any publications.

    Product Wall

    Application Immunocytochemistry/ Immunofluorescence
    Sample Rat Cell (neuron)
    Permeabilization Yes - 0.2%Triton
    Specification neuron
    Blocking step Serum as blocking agent for 40 minute(s) · Concentration: 5% · Temperature: 22°C
    Fixative Formaldehyde
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    提交于 Oct 13 2015

    Application Immunocytochemistry/ Immunofluorescence
    Sample Monkey Cell (COS7)
    Permeabilization Yes - 0.2%Triton
    Specification COS7
    Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 2% · Temperature: 22°C
    Fixative Formaldehyde
    Username

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    Verified customer

    提交于 Oct 07 2015

    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application Western blot
    Sample Mouse Cell lysate - whole cell (brain)
    Loading amount 40 µg
    Specification brain
    Gel Running Conditions Reduced Denaturing
    Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5%
    Username

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    Verified customer

    提交于 Jun 09 2011

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"