兔多克隆抗体to EN1 / Engrailed 1
Mouse, Chicken, Zebrafish
Synthetic peptide designed within residues: LMGSANGGPV VKTDSQQPLV WPAWVYCTRY SDRPSSGPRT RKLKKKKNEK , corresponding to C terminal amino acids 253-302 of Human EN1/ Engailed 1 (NP_001417)
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
Constituents: 2% Sucrose, PBS
Concentration information loading...
Immunogen affinity purified
ab83693 is purified by a peptide affinity chromatography method.
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in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use a concentration of 1 µg/ml. Predicted molecular weight: 40 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
Use at an assay dependent concentration.
Titre using peptide based assay: 1/312500.
Belongs to the engrailed homeobox family.
Contains 1 homeobox DNA-binding domain.
Information by UniProt
En 1 antibody
Engrailed 1 antibody
Western blot - Anti-EN1 / Engrailed 1 antibody (ab83693)
Anti-EN1 / Engrailed 1 antibody (ab83693) at 1 µg/ml + HepG2 cell lysate at 10 µg
HRP conjugated anti-Rabbit IgG at 1/50000 dilution
Predicted band size:
Observed band size:
37 kDa (
why is the actual band size different from the predicted?
Additional bands at:
24 kDa. We are unsure as to the identity of these extra bands.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"