Anti-EMX2抗体(ab94713)
Key features and details
- Rabbit polyclonal to EMX2
- Suitable for: WB
- Reacts with: Mouse, Human
- Isotype: IgG
概述
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产品名称
Anti-EMX2抗体
参阅全部 EMX2 一抗 -
描述
兔多克隆抗体to EMX2 -
宿主
Rabbit -
经测试应用
适用于: WBmore details -
种属反应性
与反应: Mouse, Human
预测可用于: Rat, Chicken, Cow, Dog, Pig, Zebrafish -
免疫原
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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阳性对照
- WB: Mouse brain and hippocampus tissue lysates.
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
存储溶液
pH: 7.40
Preservative: 0.02% Sodium azide
Constituent: PBS
Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help. -
Concentration information loading...
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纯度
Immunogen affinity purified -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab94713于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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WB | (1) |
Use a concentration of 1 µg/ml. Detects a band of approximately 28 kDa (predicted molecular weight: 28 kDa).
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说明 |
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WB
Use a concentration of 1 µg/ml. Detects a band of approximately 28 kDa (predicted molecular weight: 28 kDa). |
靶标
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功能
Transcription factor, which in cooperation with EMX2, acts to generate the boundary between the roof and archipallium in the developing brain. May function in combinations with OTX1/2 to specify cell fates in the developing central nervous system. -
组织特异性
Cerebral cortex. -
疾病相关
Defects in EMX2 are the cause of schizencephaly (SCHZC) [MIM:269160]. Schizencephaly is an extremely rare human congenital disorder characterized by a full-thickness cleft within the cerebral hemispheres. These clefts are lined with gray matter and most commonly involve the parasylvian regions. Large portions of the cerebral hemispheres may be absent and replaced by cerebro-spinal fluid. -
序列相似性
Belongs to the EMX homeobox family.
Contains 1 homeobox DNA-binding domain. -
细胞定位
Nucleus. - Information by UniProt
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数据库链接
- Entrez Gene: 523601 Cow
- Entrez Gene: 2018 Human
- Entrez Gene: 13797 Mouse
- Entrez Gene: 499380 Rat
- Omim: 600035 Human
- SwissProt: Q17R00 Cow
- SwissProt: Q04743 Human
- SwissProt: Q04744 Mouse
see all -
别名
- Empty spiracles homeobox 2 antibody
- Empty spiracles homolog 2 (Drosophila) antibody
- Empty spiracles homolog 2 antibody
see all
图片
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All lanes : Anti-EMX2 antibody (ab94713) at 1 µg/ml
Lane 1 : Mouse Hippocampus Tissue Lysate
Lane 2 : Brain (Mouse) Tissue Lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 28 kDa
Additional bands at: 260 kDa (possible non-specific binding), 76 kDa (possible non-specific binding)
Exposure time: 16 minutes -
All lanes : Anti-EMX2 antibody (ab94713) at 1 µg/ml
Lane 1 : Human Brain lysate
Lane 2 : Mouse Brain lysate
Lane 3 : NIH 3T3 whole cell lysate
Lane 4 : HepG2 whole cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) at 1/50000 dilution
Predicted band size: 28 kDa
Observed band size: 28 kDa
Exposure time: 1 minuteBlocking buffer: 2% BSA
Gel type: MES
Additional bands: 15 kDa (possible non-specific binding), 75 kDa (possible non-specific binding)
实验方案
数据表及文件
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SDS download
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Datasheet download
文献 (2)
ab94713 被引用在 2 文献中.
- Rosebrock D et al. Enhanced cortical neural stem cell identity through short SMAD and WNT inhibition in human cerebral organoids facilitates emergence of outer radial glial cells. Nat Cell Biol 24:981-995 (2022). PubMed: 35697781
- Rysenkova KD et al. Identification of a Novel Small RNA Encoded in the Mouse Urokinase Receptor uPAR Gene (Plaur) and Its Molecular Target Mef2d. Front Mol Neurosci 15:865858 (2022). PubMed: 35875662