概述

  • 产品名称
  • 描述
    兔多克隆抗体to Emx1
  • 经测试应用
    适用于: WBmore details
  • 种属反应性
    与反应: Human
    预测可用于: Mouse, Rat, Rabbit, Horse, Chicken, Guinea pig, Cow, Cat, Dog, Caenorhabditis elegans, Drosophila melanogaster, Zebrafish
  • 免疫原

    Synthetic peptide corresponding to a region within the internal sequence amino acids 180-229 (DGLLLHGPFA RKPKRIRTAF SPSQLLRLER AFEKNHYVVG AERKQLAGSL) of Human Emx1 (NP_004088).

  • 阳性对照
    • HepG2 cell lysate

性能

应用

Our Abpromise guarantee covers the use of ab82901 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use a concentration of 1 µg/ml. Detects a band of approximately 35 kDa (predicted molecular weight: 29 kDa). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.

靶标

图片

  • Anti-Emx1 antibody (ab82901) at 1 µg/ml + HepG2 cell lysate at 10 µg

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size : 29 kDa
    Observed band size : 35 kDa (why is the actual band size different from the predicted?)

实验方案

文献

ab82901 has not yet been referenced specifically in any publications.

客户评价及客户问答

Abcam has not validated the combination of species/application used in this Abreview.
Application
Immunohistochemistry (Frozen sections)
Sample
Zebra finch Tissue sections (embryonal forebrain)
Specification
embryonal forebrain
Fixative
Paraformaldehyde
Permeabilization
Yes - 0.1TX100 in PBS
Blocking step
Roti-Immunoblock as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 4°C
Username

Dr. Robert Storm

Verified customer

提交于 Feb 06 2012

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Human Tissue lysate - whole (Brain)
Loading amount
30 µg
Specification
Brain
Gel Running Conditions
Reduced Denaturing (4-12%)
Blocking step
LiCore Blocking Buffer as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 100% · Temperature: 25°C
Username

Abcam user community

Verified customer

提交于 Jun 30 2011

Abcam has not validated the combination of species/application used in this Abreview.
Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (Brain)
Specification
Brain
Fixative
Paraformaldehyde
Permeabilization
Yes - Triton X-100
Blocking step
Serum as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 25°C
Username

Abcam user community

Verified customer

提交于 Jun 09 2011

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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