概述

  • 产品名称
    Anti-EMSY抗体
    参阅全部 EMSY 一抗
  • 描述
    山羊多克隆抗体to EMSY
  • 宿主
    Goat
  • 特异性
    This antibody detects a band of the same size by Western blot as ab123, which was raised with a recombinant fragment of EMSY with no sequence in common with the immunogen for this antibody (and was raised 7 years previous to this antibody). This confirms that the band detected is EMSY.
  • 经测试应用
    适用于: ICC/IF, WB, ICCmore details
  • 种属反应性
    与反应: Human
  • 免疫原

    Recombinant fragment corresponding to EMSY ENT domain:

    MPVVWPTLLDLSRDECKRILRKLELEAYAGVISALRAQGDLTKEKKDLLG ELSKVLSISTER HRAEVRRAVNDERLTTIAHNMSGPNSSSEWSIEGRRL

    , corresponding to amino acids 1-100 of Human EMSY - FPLC purified.

  • 阳性对照
    • MCF-7 cells.

性能

应用

Our Abpromise guarantee covers the use of ab4579 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ICC/IF Use at an assay dependent dilution.
WB 1/500. Predicted molecular weight: 141 kDa.
ICC Use at an assay dependent dilution.

靶标

图片

  • Western blot - Anti-EMSY antibody (ab4579)
    Western blot - Anti-EMSY antibody (ab4579)
    Lanes 1-2 : Anti-EMSY antibody (ab4579) at 1 µg/ml
    Lane 3 : Anti-EMSY antibody (ab123) at 1 µg/ml

    All lanes : MCF7 (Human breast adenocarcinoma cell line) Whole Cell Lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    Lanes 1-2 : Rabbit polyclonal to Goat IgG - H&L - Pre-Adsorbed (HRP) at 1/10000 dilution
    Lane 3 : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/50000 dilution

    Developed using the ECL technique.

    Performed under reducing conditions.

    Predicted band size: 141 kDa
    Observed band size: 100 kDa (why is the actual band size different from the predicted?)
    Additional bands at: 37 kDa, 70 kDa. We are unsure as to the identity of these extra bands.


    Exposure time: 2 minutes


    Western blot of EMSY on MCF-7 cell lysate.

    Lane 1: ab4579 at 1ug/ml with 2% BSA blocking.

    Lane 2: ab4579 at 1ug/ml with 3% milk blocking.

    Lane 3: ab123 at 1ug/ml with 2% BSA blocking.

    Variability in the size at which EMSY runs by Western blot has been experienced with different protein marker systems. EMSY has been observed to run at between 115 and 150kD (even though the predicted size is 141kD). However, that the same size band is detected by both ab123 and ab4579 (raised with immunogens of different sequence) confirms the specificity of these antibodies.

     Lane 1 & 2 - Exposure time: 2 min.

    Lane 3 - Exposure time: 2 sec.

文献

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