概述
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产品名称Anti-EMSY抗体
参阅全部 EMSY 一抗 -
描述山羊多克隆抗体to EMSY
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特异性This antibody detects a band of the same size by Western blot as ab123, which was raised with a recombinant fragment of EMSY with no sequence in common with the immunogen for this antibody (and was raised 7 years previous to this antibody). This confirms that the band detected is EMSY.
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经测试应用适用于: ICC/IF, WB, ICCmore details
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种属反应性与反应: Human
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免疫原
Recombinant fragment corresponding to EMSY ENT domain:
MPVVWPTLLDLSRDECKRILRKLELEAYAGVISALRAQGDLTKEKKDLLG ELSKVLSISTER HRAEVRRAVNDERLTTIAHNMSGPNSSSEWSIEGRRL
, corresponding to amino acids 1-100 of Human EMSY - FPLC purified. -
阳性对照
- MCF-7 cells.
性能
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形式Liquid
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存放说明Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
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存储溶液pH: 7.40
Preservative: 0.025% Sodium azide
Constituent: PBS
This product may contain up to 3% BSA depending on the batch. For specific batch formulations please contact us. -
Concentration information loading... -
纯度Immunogen affinity purified
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克隆多克隆
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同种型IgG
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研究领域
相关产品
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Compatible Secondaries
应用
Our Abpromise guarantee covers the use of ab4579 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| 应用 | Ab评论 | 说明 |
|---|---|---|
| ICC/IF | Use at an assay dependent dilution. | |
| WB | 1/500. Predicted molecular weight: 141 kDa. | |
| ICC | Use at an assay dependent dilution. |
靶标
- Information by UniProt
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数据库链接
- Entrez Gene: 56946 Human
- Omim: 608574 Human
- SwissProt: Q7Z589 Human
- Unigene: 352588 Human
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别名
- C11orf30 antibody
- Chromosome 11 open reading frame 30 antibody
- Emsy antibody
see all
Anti-EMSY antibody 图像
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Western blot - Anti-EMSY antibody (ab4579)Lanes 1 - 2 : Anti-EMSY antibody (ab4579) at 1 µg/ml
Lane 3 : Anti-EMSY antibody (ab123) at 1 µg/ml
Lane 1 : MCF7 (Human breast adenocarcinoma cell line) Whole Cell Lysate
Lane 2 : MCF7 (Human breast adenocarcinoma cell line) Whole Cell Lysate
Lane 3 : MCF7 (Human breast adenocarcinoma cell line) Whole Cell Lysate
Lysates/proteins at 10 µg per lane.
Secondary
Lanes 1 - 2 : Rabbit polyclonal to Goat IgG - H&L - Pre-Adsorbed (HRP) at 1/10000 dilution
Lane 3 : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/50000 dilution
Developed using the ECL technique
Performed under reducing conditions.
Predicted band size : 141 kDa
Observed band size : 100 kDa (why is the actual band size different from the predicted?)
Additional bands at : 37 kDa,70 kDa. We are unsure as to the identity of these extra bands.
Exposure time : 2 minutesWestern blot of EMSY on MCF-7 cell lysate.
Lane 1: ab4579 at 1ug/ml with 2% BSA blocking.
Lane 2: ab4579 at 1ug/ml with 3% milk blocking.
Lane 3: ab123 at 1ug/ml with 2% BSA blocking.
Variability in the size at which EMSY runs by Western blot has been experienced with different protein marker systems. EMSY has been observed to run at between 115 and 150kD (even though the predicted size is 141kD). However, that the same size band is detected by both ab123 and ab4579 (raised with immunogens of different sequence) confirms the specificity of these antibodies.
Lane 1 & 2 - Exposure time: 2 min.
Lane 3 - Exposure time: 2 sec.
Anti-EMSY antibody (ab4579)参考文献
ab4579 has not yet been referenced specifically in any publications.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"