概述

  • 产品名称Anti-EML4抗体
    参阅全部 EML4 一抗
  • 描述
    小鼠单克隆抗体to EML4
  • 经测试应用适用于: ICC/IF, IHC-P, WB, Flow Cytmore details
  • 种属反应性
    与反应: Human
  • 免疫原

    Recombinant full length protein with ~26kDa proprietary tag: MCYTPCKKYT DMNRQFLEKK EHFFKYLGNT ALSDQQGVYL RTSVTFGVAM YNEIYNHDTL RW, corresponding to amino acids 1-63 of Human EML4 protein (AAH08685.1).

性能

  • 形式Liquid
  • 存放说明Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • 存储溶液Preservative: None
    PBS, pH 7.2
  • Concentration information loading...
  • 纯度Protein G purified
  • 克隆单克隆
  • 同种型IgG1
  • 轻链类型kappa
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab57297 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ICC/IF Use a concentration of 10 µg/ml.
IHC-P Use a concentration of 5 µg/ml.
WB Use a concentration of 1 - 5 µg/ml.
Flow Cyt Use 1µg for 106 cells. ab170190-Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.

靶标

  • 功能May modify the assembly dynamics of microtubules, such that microtubules are slightly longer, but more dynamic.
  • 序列相似性Belongs to the WD repeat EMAP family.
    Contains 9 WD repeats.
  • 发展阶段Strongly overexpressed during mitosis.
  • 细胞定位Cytoplasm > cytoskeleton.
  • Information by UniProt
  • 数据库链接
  • 别名
    • EML4 antibody
    • C2orf2 antibody
    • Echinoderm microtubule associated protein like 4 antibody
    • Echinoderm microtubule-associated protein-like 4 antibody
    • ELP 120 antibody
    • ELP120 antibody
    • EMAL4_HUMAN antibody
    • EMAP 4 antibody
    • EMAP-4 antibody
    • EMAP4 antibody
    • EML 4 antibody
    • eml4 antibody
    • Restrictedly overexpressed proliferation associated protein antibody
    • Restrictedly overexpressed proliferation-associated protein antibody
    • Ropp 120 antibody
    • ROPP120 antibody
    see all

Anti-EML4 antibody 图像

  • EML4 antibody (ab57297) used in immunofluorescence at 1-5ug/ml on HeLa cells.
  • Western blot against tagged recombinant protein immunogen using ab57297 EML4 antibody at 1ug/ml. Predicted band size of immunogen is 33 kDa
  • IHC image of ab57297 staining in human breast carcinoma formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab57297, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

  • Overlay histogram showing HeLa cells stained with ab57297 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab57297, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed.

Anti-EML4 antibody (ab57297)参考文献

ab57297 has not yet been referenced specifically in any publications.

Product Wall

Abcam has not validated the combination of species/application used in this Abreview.
Application Immunocytochemistry/ Immunofluorescence
Sample Xenopus laevis Cell (meiotic egg extract)
Specification meiotic egg extract
Fixative Methanol
Permeabilization No
Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 25°C
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提交于 Mar 03 2010

Application Western blot
Sample Xenopus laevis Cell lysate - other (meiotic egg extract)
Loading amount 25 µg
Specification meiotic egg extract
Gel Running Conditions Reduced Denaturing (10%)
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
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Abcam user community

Verified customer

提交于 Feb 08 2010

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"