概述

  • 产品名称
  • 描述
    兔多克隆抗体to EML1
  • 经测试应用
    适用于: WBmore details
  • 种属反应性
    与反应: Mouse, Human
  • 免疫原

    Synthetic peptide, corresponding to a sequence within amino acids 772-834 of Human EML1 (NP_001008707).

  • 阳性对照
    • 293T, A431, H1299, HeLaS3, HepG2, MOLT4, Raji and NIH3T3 cells

性能

  • 形式
    Liquid
  • 存放说明
    Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • 存储溶液
    Preservative: 0.01% Thimerosal (merthiolate)
    Constituents: 20% Glycerol, 1% BSA, 1X PBS, pH 7.0
  • Concentration information loading...
  • 纯度
    Immunogen affinity purified
  • 克隆
    多克隆
  • 同种型
    IgG
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab97342 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/500 - 1/3000. Predicted molecular weight: 90 kDa.

靶标

  • 功能
    May modify the assembly dynamics of microtubules, such that microtubules are slightly longer, but more dynamic.
  • 组织特异性
    Ubiquitous; expressed in most tissues with the exception of thymus and peripheral blood lymphocytes.
  • 序列相似性
    Belongs to the WD repeat EMAP family.
    Contains 10 WD repeats.
  • 细胞定位
    Cytoplasm > cytoskeleton.
  • Information by UniProt
  • 数据库链接
  • 别名
    • echinoderm microtubule associated protein like 1 antibody
    • Echinoderm microtubule-associated protein-like 1 antibody
    • ELP79 antibody
    • EMAL1_HUMAN antibody
    • EMAP antibody
    • EMAP-1 antibody
    • EMAPL antibody
    • Eml1 antibody
    • FLJ45033 antibody
    • HuEMAP antibody
    • HuEMAP-1 antibody
    see all

图片

  • Anti-EML1 antibody (ab97342) at 1/500 dilution + H1299 whole cell lysate at 30 µg

    Predicted band size : 90 kDa

实验方案

文献

ab97342 has not yet been referenced specifically in any publications.

客户评价及客户问答

Abcam has not validated the combination of species/application used in this Abreview.
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Mouse Tissue sections (retina)
Specification
retina
Fixative
Paraformaldehyde
Antigen retrieval step
None
Permeabilization
No
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
Username

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提交于 Apr 16 2013

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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