概述

  • 产品名称Anti-EMILIN1抗体
    参阅全部 EMILIN1 一抗
  • 描述
    兔多克隆抗体to EMILIN1
  • 经测试应用适用于: WBmore details
  • 种属反应性
    与反应: Human
    预测可用于: Rabbit, Cow, Dog, Pig, Chimpanzee, Macaque Monkey, Gorilla, Orangutan
  • 免疫原

    Synthetic peptide conjugated to KLH derived from within residues 450 - 550 of Human EMILIN1.

  • 阳性对照
    • This antibody gave a positive signal in the following tissue lysates: Human Small Intestine; Human Cervix; Human Fetal Heart; Human Fetal Kidney.

性能

应用

Our Abpromise guarantee covers the use of ab125860 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/250. Detects a band of approximately 130 kDa (predicted molecular weight: 106 kDa).

靶标

  • 功能May be responsible for anchoring smooth muscle cells to elastic fibers, and may be involved not only in the formation of the elastic fiber, but also in the processes that regulate vessel assembly. Has cell adhesive capacity.
  • 组织特异性Distributed in tissues where resilience and elastic recoil are prominent. Highest levels in the adult small intestine, aorta, lung, uterus, and appendix and in the fetal spleen, kidney, lung, and heart; intermediate expression was detected in adult liver, ovary, colon, stomach, lymph node and spleen; adult heart, bladder, prostate, adrenal gland, mammary gland, placenta and kidney showed low expression whereas a series of other adult tissues, including skeletal muscle and different regions of adult brain show no expression.
  • 序列相似性Contains 1 C1q domain.
    Contains 1 collagen-like domain.
    Contains 1 EMI domain.
  • 细胞定位Secreted > extracellular space > extracellular matrix. Found mainly at the interface between amorphous elastin and microfibrils.
  • Information by UniProt
  • 数据库链接
  • 别名
    • Elastin microfibril interface located protein 1 antibody
    • Elastin microfibril interface located protein antibody
    • Elastin microfibril interface-located protein 1 antibody
    • Elastin microfibril interfacer 1 antibody
    • EMIL1_HUMAN antibody
    • EMILIN 1 antibody
    • EMILIN antibody
    • EMILIN-1 antibody
    • Emilin1 antibody
    • gp115 antibody
    see all

Anti-EMILIN1 antibody 图像

  • All lanes : Anti-EMILIN1 antibody (ab125860) at 1/250 dilution

    Lane 1 : Human small intestine tissue lysate - total protein (ab29276)
    Lane 2 : Human cervix normal tissue lysate - total protein (ab29226)
    Lane 3 : Kidney (Human) Tissue Lysate - fetal normal tissue (ab30204)
    Lane 4 : Heart (Human) Whole Cell Lysate - fetal normal tissue

    Lysates/proteins at 10 µg per lane.

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/10000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 106 kDa
    Observed band size : 130 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 160 kDa. We are unsure as to the identity of these extra bands.

    Exposure time : 90 seconds

Anti-EMILIN1 antibody (ab125860)参考文献

ab125860 has not yet been referenced specifically in any publications.

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