Anti-Embigin homolog抗体(ab72195)

概述

  • 产品名称Anti-Embigin homolog抗体
    参阅全部 Embigin homolog 一抗
  • 描述
    兔多克隆抗体to Embigin homolog
  • 经测试应用适用于: WBmore details
  • 种属反应性
    与反应: Mouse, Human
  • 免疫原

    Synthetic peptide corresponding to a portion of amino acids 130-180 of Human Embigin homolog

  • 阳性对照
    • MCF7 and Raw cell lysates.

性能

相关产品

应用

Our Abpromise guarantee covers the use of ab72195 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use a concentration of 0.25 - 0.5 µg/ml. Detects a band of approximately 37 kDa (predicted molecular weight: 37 kDa).

靶标

Anti-Embigin homolog antibody 图像

  • All lanes : Anti-Embigin homolog antibody (ab72195) at 0.25 µg/ml

    Lane 1 : MCF7 cell lysate
    Lane 2 : MCF7 cell lysate with immunising peptide
    Lane 3 : Raw cell lysate


    Predicted band size : 37 kDa
    Observed band size : 37 kDa

Anti-Embigin homolog antibody (ab72195)参考文献

ab72195 has not yet been referenced specifically in any publications.

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Human Cell lysate - whole cell (HUES7 ES cells)
Gel Running Conditions Reduced Denaturing
Loading amount 20 µg
Specification HUES7 ES cells
Blocking step Milk as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 16°C
Username

Abcam user community

Verified customer

提交于 Feb 02 2016

Application Immunocytochemistry/ Immunofluorescence
Sample Human Cell (HEPG2)
Specification HEPG2
Blocking step Serum as blocking agent for 30 minute(s) · Concentration: 1% · Temperature: rt°C
Fixative Paraformaldehyde
Username

Abcam user community

Verified customer

提交于 Nov 18 2015

Application Western blot
Sample Human Cell lysate - whole cell (HEPG2)
Gel Running Conditions Reduced Denaturing (12.5)
Loading amount 20 µg
Specification HEPG2
Blocking step Milk as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 16°C
Username

Abcam user community

Verified customer

提交于 Nov 13 2015

Application Western blot
Sample Human Cell lysate - whole cell (human embryonic stem cells)
Gel Running Conditions Reduced Denaturing (10% gel)
Loading amount 100000 cells
Specification human embryonic stem cells
Blocking step Milk as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 16°C
Username

Abcam user community

Verified customer

提交于 Aug 18 2015

Application Immunocytochemistry/ Immunofluorescence
Blocking step Serum as blocking agent for 30 minute(s) · Concentration: 1% · Temperature: 16°C
Sample Mouse Cell (Mouse embryonic stem cell)
Specification Mouse embryonic stem cell
Permeabilization Yes - 0.1% Triton X
Fixative methanol:acetone 1:1
Username

Abcam user community

Verified customer

提交于 Sep 23 2014

Application Western blot
Loading amount 100000 cells
Gel Running Conditions Reduced Denaturing (10% gel)
Sample Mouse Cell lysate - whole cell (mouse embryonic stem cell)
Specification mouse embryonic stem cell
Blocking step Milk as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 16°C
Username

Abcam user community

Verified customer

提交于 Sep 17 2014

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"