概述

  • 产品名称Anti-EMAP II抗体
    参阅全部 EMAP II 一抗
  • 描述
    兔多克隆抗体to EMAP II
  • 经测试应用适用于: WB, ICC/IF, IPmore details
  • 种属反应性
    与反应: Human
    预测可用于: Pig
  • 免疫原

    Recombinant fragment, corresponding to a region within the N terminal amino acids 1-233 of Human EMAP II.

  • 阳性对照
    • H1299, HeLa S3, HepG2 cells, HeLa cell lysates

性能

应用

Our Abpromise guarantee covers the use of ab96506 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/500 - 1/3000. Predicted molecular weight: 34 kDa.
ICC/IF 1/100 - 1/200.
IP Use at an assay dependent concentration. PubMed: 24312579

靶标

  • 功能Non-catalytic component of the multisynthase complex. Stimulates the catalytic activity of cytoplasmic arginyl-tRNA synthase. Binds tRNA. Possesses inflammatory cytokine activity. Negatively regulates TGF-beta signaling through stabilization of SMURF2 by binding to SMURF2 and inhibiting its SMAD7-mediated degradation. Involved in glucose homeostasis through induction of glucagon secretion at low glucose levels. Promotes dermal fibroblast proliferation and wound repair. Regulates KDELR1-mediated retention of HSP90B1/gp96 in the endoplasmic reticulum. Plays a role in angiogenesis by inducing endothelial cell migration at low concentrations and endothelian cell apoptosis at high concentrations. Induces maturation of dendritic cells and monocyte cell adhesion. Modulates endothelial cell responses by degrading HIF-1A through interaction with PSMA7.
  • 疾病相关Defects in AIMP1 are the cause of leukodystrophy hypomyelinating type 3 (HLD3) [MIM:260600]. A severe autosomal recessive hypomyelinating leukodystrophy characterized by early infantile onset of global developmental delay, lack of development, lack of speech acquisition, and peripheral spasticity associated with decreased myelination in the central nervous system.
  • 序列相似性Contains 1 tRNA-binding domain.
  • 翻译后修饰Cleaved by caspase-7 in response to apoptosis to produce EMAP-II.
  • 细胞定位Nucleus. Cytoplasm > cytosol. Cytoplasmic vesicle > secretory vesicle. Secreted. Endoplasmic reticulum. Golgi apparatus. Enriched in secretory vesicles of pancreatic alpha cells and secreted from the pancreas in response to low glucose levels (By similarity). Also secreted in response to hypoxia and both apoptotic and necrotic cell death.
  • Information by UniProt
  • 数据库链接
  • 别名
    • AIMP 1 antibody
    • Aimp1 antibody
    • AIMP1/p43 antibody
    • AIMP1_HUMAN antibody
    • Aminoacyl tRNA synthetase complex-interacting multifunctional protein 1 antibody
    • ARS interacting multifunctional protein 1 antibody
    • EMAP 2 antibody
    • EMAP-2 antibody
    • EMAP-II antibody
    • EMAP2 antibody
    • EMAPII antibody
    • Endothelial monocyte activating polypeptide 2 antibody
    • Endothelial monocyte activating polypeptide antibody
    • Endothelial monocyte-activating polypeptide 2 antibody
    • Endothelial monocyte-activating polypeptide II antibody
    • HLD3 antibody
    • Multisynthase complex auxiliary component p43 antibody
    • Multisynthetase complex auxiliary component p43 antibody
    • OTTHUMP00000219673 antibody
    • p43 antibody
    • Scye 1 antibody
    • Scye1 antibody
    • Scye1, formerly antibody
    • Small inducible cytokine subfamily E member 1 antibody
    • Small inducible cytokine subfamily E, member 1 (endothelial monocyte-activating) antibody
    • Small inducible cytokine subfamily E, member 1, formerly antibody
    see all

Anti-EMAP II antibody 图像

  • All lanes : Anti-EMAP II antibody (ab96506) at 1/1000 dilution

    Lane 1 : H1299 whole cell lysate
    Lane 2 : HepG2 whole cell lysate

    Lysates/proteins at 30 µg per lane.


    Predicted band size : 34 kDa
    12% SDS PAGE
  • Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using ab96506 at 1:200 dilution. Image 2: Merged with DNA probe.

Anti-EMAP II antibody (ab96506)参考文献

This product has been referenced in:
  • Park SJ  et al. Evaluation of Multi-tRNA Synthetase Complex by Multiple Reaction Monitoring Mass Spectrometry Coupled with Size Exclusion Chromatography. PLoS One 10:e0142253 (2015). WB ; Human . Read more (PubMed: 26544075) »
  • Kim K  et al. Reinvestigation of aminoacyl-TRNA synthetase core complex by affinity purification-mass spectrometry reveals TARSL2 as a potential member of the complex. PLoS One 8:e81734 (2013). WB, IP ; Human . Read more (PubMed: 24312579) »

See all 2 Publications for this product

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