概述

  • 产品名称Anti-EMAP II抗体[546-2]
    参阅全部 EMAP II 一抗
  • 描述
    小鼠单克隆抗体[546-2] to EMAP II
  • 经测试应用适用于: IHC-Fr, IHC-P, ELISA, WBmore details
  • 种属反应性
    与反应: Rat, Human
  • 免疫原

    Recombinant human EMAP II

性能

应用

Our Abpromise guarantee covers the use of ab15693 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
IHC-Fr 1/40.
IHC-P 1/40. Perform heat mediated antigen retrieval via the microwave method before commencing with IHC staining protocol.
ELISA Use at an assay dependent dilution.
WB Use at an assay dependent dilution. Predicted molecular weight: 34 kDa.

靶标

  • 功能Non-catalytic component of the multisynthase complex. Stimulates the catalytic activity of cytoplasmic arginyl-tRNA synthase. Binds tRNA. Possesses inflammatory cytokine activity. Negatively regulates TGF-beta signaling through stabilization of SMURF2 by binding to SMURF2 and inhibiting its SMAD7-mediated degradation. Involved in glucose homeostasis through induction of glucagon secretion at low glucose levels. Promotes dermal fibroblast proliferation and wound repair. Regulates KDELR1-mediated retention of HSP90B1/gp96 in the endoplasmic reticulum. Plays a role in angiogenesis by inducing endothelial cell migration at low concentrations and endothelian cell apoptosis at high concentrations. Induces maturation of dendritic cells and monocyte cell adhesion. Modulates endothelial cell responses by degrading HIF-1A through interaction with PSMA7.
  • 疾病相关Defects in AIMP1 are the cause of leukodystrophy hypomyelinating type 3 (HLD3) [MIM:260600]. A severe autosomal recessive hypomyelinating leukodystrophy characterized by early infantile onset of global developmental delay, lack of development, lack of speech acquisition, and peripheral spasticity associated with decreased myelination in the central nervous system.
  • 序列相似性Contains 1 tRNA-binding domain.
  • 翻译后修饰Cleaved by caspase-7 in response to apoptosis to produce EMAP-II.
  • 细胞定位Nucleus. Cytoplasm > cytosol. Cytoplasmic vesicle > secretory vesicle. Secreted. Endoplasmic reticulum. Golgi apparatus. Enriched in secretory vesicles of pancreatic alpha cells and secreted from the pancreas in response to low glucose levels (By similarity). Also secreted in response to hypoxia and both apoptotic and necrotic cell death.
  • Information by UniProt
  • 数据库链接
  • 别名
    • AIMP 1 antibody
    • Aimp1 antibody
    • AIMP1/p43 antibody
    • AIMP1_HUMAN antibody
    • Aminoacyl tRNA synthetase complex-interacting multifunctional protein 1 antibody
    • ARS interacting multifunctional protein 1 antibody
    • EMAP 2 antibody
    • EMAP-2 antibody
    • EMAP-II antibody
    • EMAP2 antibody
    • EMAPII antibody
    • Endothelial monocyte activating polypeptide 2 antibody
    • Endothelial monocyte activating polypeptide antibody
    • Endothelial monocyte-activating polypeptide 2 antibody
    • Endothelial monocyte-activating polypeptide II antibody
    • HLD3 antibody
    • Multisynthase complex auxiliary component p43 antibody
    • Multisynthetase complex auxiliary component p43 antibody
    • OTTHUMP00000219673 antibody
    • p43 antibody
    • Scye 1 antibody
    • Scye1 antibody
    • Scye1, formerly antibody
    • Small inducible cytokine subfamily E member 1 antibody
    • Small inducible cytokine subfamily E, member 1 (endothelial monocyte-activating) antibody
    • Small inducible cytokine subfamily E, member 1, formerly antibody
    see all

Anti-EMAP II antibody [546-2] 图像

  • Lanes 1, 2, 3, 4, 6, 7, 8 & 9 : Anti-EMAP II antibody [546-2] (ab15693) at 1/1000 dilution
    Lane 5 :
    Lane 10 :

    Lane 1 : HeLa Cytoplasmic Extract (0.625ug)
    Lane 2 : HeLa Cytoplasmic Extract (1.25ug)
    Lane 3 : HeLa Cytoplasmic Extract (2.5ug)
    Lane 4 : HeLa Cytoplasmic Extract (5ug)
    Lane 5 : High Range Rainbow MWMs
    Lane 6 : HeLa Nuclear Extract (0.625ug)
    Lane 7 : HeLa Nuclear Extract (1.25ug)
    Lane 8 : HeLa Nuclear Extract (2.5ug)
    Lane 9 : HeLa Nuclear Extract (5ug)
    Lane 10 : High Range Rainbow MWMs

    Secondary
    Lanes 1, 2, 3, 4, 6, 7, 8 & 9 : HRP conjugated Sheep anti-mouse IgG


    Predicted band size : 34 kDa
    Observed band size : 43 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 50 kDa (possible non-specific binding),66 kDa (possible non-specific binding),80 kDa (possible non-specific binding).

    This image is courtesy of an anonymous Abreview

    See Abreview

Anti-EMAP II antibody [546-2] (ab15693)参考文献

This product has been referenced in:
  • Pechenino AS  et al. Impact of aging vs. estrogen loss on cardiac gene expression: estrogen replacement and inflammation. Physiol Genomics 43:1065-73 (2011). WB ; Rat . Read more (PubMed: 21750230) »
  • Marik C  et al. Lesion genesis in a subset of patients with multiple sclerosis: a role for innate immunity? Brain 130:2800-15 (2007). ICC/IF . Read more (PubMed: 17956913) »

See all 2 Publications for this product

Product Wall

Application Western blot
Sample Rat Tissue lysate - whole (Heart--Left ventricle)
Loading amount 40 µg
Specification Heart--Left ventricle
Gel Running Conditions Reduced Denaturing
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Dr. Angela Pechenino

Verified customer

提交于 Mar 19 2010

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Human Cell lysate - other (HeLa Cytoplasmic Extract; HeLa Nuclear Extract)
Loading amount 5 µg
Specification HeLa Cytoplasmic Extract; HeLa Nuclear Extract
Blocking step Milk as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 10%
Username

Abcam user community

Verified customer

提交于 Nov 16 2006

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"