Synthetic peptide (Unfortunately the amino acid sequence is considered to be commercially sensitive). The peptide sequence is within the region between aa2220 and aa2302 of human ELYS using the numbering given in entry AAN65622.1 (GeneID 25909).
HeLa cells nuclear extract.
存放说明Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
应用说明IP: Use 2 to 5 ug/mg of lysate.
WB: 1/2000 - 1/10000. Predicted molecular weight: 272 kDa.
Not tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
Prepare working dilution immediately before use.
功能Required for the assembly of a functional nuclear pore complex (NPC) on the surface of chromosomes as nuclei form at the end of mitosis. May initiate NPC assembly by binding to chromatin and recruiting the Nup107-160 subcomplex of the NPC. Also required for the localization of the Nup107-160 subcomplex of the NPC to the kinetochore during mitosis and for the completion of cytokinesis.
序列相似性Belongs to the ELYS family. Contains 1 A.T hook DNA-binding domain.
翻译后修饰Phosphorylated upon DNA damage, probably by ATM or ATR.
细胞定位Cytoplasm. Nucleus matrix. Chromosome > centromere > kinetochore. Nucleus > nucleoplasm. Nucleus > nuclear pore complex. Localizes to the nuclear pore complex (NPC) throughout interphase. Localizes to the kinetochore from prophase, and this appears to require the Nup107-160 subcomplex of the NPC. Localizes to the periphery of chromatin from late anaphase.