概述

  • 产品名称Anti-ELOVL6抗体
    参阅全部 ELOVL6 一抗
  • 描述
    兔多克隆抗体to ELOVL6
  • 经测试应用适用于: IHC-P, ICC/IF, WBmore details
  • 种属反应性
    与反应: Mouse, Rat, Human
  • 免疫原

    A 16 amino acid peptide near the carboxy terminus of the human ELOVL6 (GenBank accession no. NP_076995).

  • 阳性对照
    • Human brain tissue lysate.

性能

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应用

Our Abpromise guarantee covers the use of ab69857 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
IHC-P Use a concentration of 5 µg/ml.
ICC/IF Use a concentration of 1 µg/ml.
WB Use a concentration of 1 - 2 µg/ml. Detects a band of approximately 28 kDa (predicted molecular weight: 31 kDa).Can be blocked with ELOVL6 peptide (ab114079).

靶标

  • 功能Condensing enzyme that catalyzes the synthesis of saturated and monounsaturated fatty acids. Highest activity toward C16:0 acyl-CoAs.
  • 组织特异性Ubiquitous.
  • 序列相似性Belongs to the ELO family.
  • 翻译后修饰N-Glycosylated.
  • 细胞定位Endoplasmic reticulum membrane.
  • Information by UniProt
  • 数据库链接
  • 别名
    • 3-keto acyl-CoA synthase ELOVL6 antibody
    • Elongation of very long chain fatty acids protein 6 antibody
    • ELOV6_HUMAN antibody
    • ELOVL family member 6, elongation of long chain fatty acids (FEN1/Elo2, SUR4/Elo3 like, yeast) antibody
    • ELOVL fatty acid elongase 6 antibody
    • ELOVL6 antibody
    • FACE antibody
    • FAE antibody
    • Fatty acid elongase 2 antibody
    • Fatty acyl CoA elongase antibody
    • Fatty acyl-CoA elongase antibody
    • FLJ23378 antibody
    • hELO2 antibody
    • LCE antibody
    • Long chain fatty acyl elongase antibody
    • Long-chain fatty-acyl elongase antibody
    • MGC5487 antibody
    • Myelin associated SUR4 protein antibody
    see all

Anti-ELOVL6 antibody 图像

  • Lane 1 : Anti-ELOVL6 antibody (ab69857) at 1 µg/ml
    Lane 2 : Anti-ELOVL6 antibody (ab69857) at 2 µg/ml

    Lane 1 : Human brain tissue lysate
    Lane 2 : Human brain tissue lysate

    Lysates/proteins at 15 µg per lane.


    Predicted band size : 31 kDa
    Observed band size : 28 kDa (why is the actual band size different from the predicted?)
  • Immunohistochemistry of ELOVL6 in human brain with ab69857 at 5 μg/ml.
  • ICC/IF image of ab69857 stained PC12 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab69857, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

  • Immunofluorescence of ELOVL6 in Human Brain cells using ab69857 at 20 ug/ml.

Anti-ELOVL6 antibody (ab69857)参考文献

This product has been referenced in:
  • Marks KA  et al. Hepatic and plasma sex differences in saturated and monounsaturated fatty acids are associated with differences in expression of elongase 6, but not stearoyl-CoA desaturase in Sprague-Dawley rats. Genes Nutr 8:317-27 (2013). WB ; Rat . Read more (PubMed: 23180365) »
  • Chamulitrat W  et al. Ursodeoxycholyl lysophosphatidylethanolamide inhibits lipoapoptosis by shifting fatty acid pools toward monosaturated and polyunsaturated fatty acids in mouse hepatocytes. Mol Pharmacol 84:696-709 (2013). Mouse . Read more (PubMed: 23974795) »

See all 3 Publications for this product

Product Wall

Thank you very much for your call today and for letting us know about the trouble with this antibody.

As we discussed, I'm sending a free of charge replacement vial on the order *** which should arrive tomorrow.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"