Synthetic peptide: corresponding to amino acids 249-299 Y IQTYNKKG AS RRKDHLK DHQ NGSMAA VNGH TNSFS PLENN VKPR KLRKD of Human ELOVL5
Human brain tissue; MCF7 cell extract.
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
Preservative: 0.02% Sodium azide Constituents: PBS, 50% Glycerol, 0.88% Sodium chloride Note: PBS (without Mg2+ and Ca2+)
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Immunogen affinity purified
ab109983 was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
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in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/500 - 1/1000. Predicted molecular weight: 35 kDa.
1/50 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Condensing enzyme that catalyzes the synthesis of monounsaturated and of polyunsaturated very long chain fatty acids Acts specifically toward polyunsaturated acyl-CoA with the higher activity toward C18:3(n-6) acyl-CoA.
Ubiquitous. Highly expressed in the adrenal gland and testis. Weekly expressed in prostate, lung and brain.
Belongs to the ELO family.
Endoplasmic reticulum membrane.
Information by UniProt
3 keto acyl CoA synthase ELOVL5 antibody
3-keto acyl-CoA synthase Elovl5 antibody
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - ELOVL5 antibody (ab109983)
ab109983 at 1/50 dilution staining ELOVL5 in paraffin-embedded Human brain tissue by Immunohistochemistry. The image on the right is treated with the synthesized peptide.
Western blot - ELOVL5 antibody (ab109983)
All lanes : Anti-ELOVL5 antibody (ab109983) at 1/500 dilution Lane 1 : MCF7 cell extract Lane 2 : MCF7 cell extract with immunizing peptide at 10 µg Lysates/proteins at 30 µg per lane. Predicted band size : 35 kDa
has not yet been referenced specifically in any publications.
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