概述

  • 产品名称Anti-Elongin A抗体
    参阅全部 Elongin A 一抗
  • 描述
    小鼠多克隆抗体to Elongin A
  • 经测试应用适用于: WBmore details
  • 种属反应性
    与反应: Saccharomyces cerevisiae
  • 免疫原

    Fusion protein:

    VKDIRNNKYRIPYRMLYSKYQQEVEKKQEESAERLRLEMQKLQQEREKKQ TIVVDHTVYFKKRNTKKTTRLHNEPHSQLYMKSLKDHESRLKHFKDGGFN

    , corresponding to amino acids 115/214 of S. cerevisiae Elongin A

  • 常规说明Produced from outbred CD1 mice


    This antibody was raised by a genetic immunization technique. Genetic immunization can be used to generate antibodies by directly delivering antigen-coding DNA into the animal, rather than injecting a protein or peptide (Tang et al. PubMed: 1545867; Chambers and Johnston PubMed: 12910245; Barry and Johnston PubMed: 9234514). The animal`s cells produce the protein, which stimulates the animal`s immune system to produce antibodies against that particular protein. A vector coding for a partial fusion protein was used for genetic immunisation of a mouse and the resulting serum was tested in Western blot against an E.coli lysate containing that partial fusion protein. Genetic immunization offers enormous advantages over the traditional protein-based immunization method. DNA is faster, cheaper and easier to produce and can be produced by standard techniques readily amenable to automation. Furthermore, the antibodies generated by genetic immunization are usually of superior quality with regard to specificity, affinity and recognizing the native protein.

性能

  • 形式Liquid
  • 存放说明Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term.
  • 存储溶液Constituents: 50% Glycerol
  • 纯度Whole antiserum
  • Primary antibody说明This antibody was raised by a genetic immunization technique. Genetic immunization can be used to generate antibodies by directly delivering antigen-coding DNA into the animal, rather than injecting a protein or peptide (Tang et al. PubMed: 1545867; Chambers and Johnston PubMed: 12910245; Barry and Johnston PubMed: 9234514). The animal`s cells produce the protein, which stimulates the animal`s immune system to produce antibodies against that particular protein. A vector coding for a partial fusion protein was used for genetic immunisation of a mouse and the resulting serum was tested in Western blot against an E.coli lysate containing that partial fusion protein. Genetic immunization offers enormous advantages over the traditional protein-based immunization method. DNA is faster, cheaper and easier to produce and can be produced by standard techniques readily amenable to automation. Furthermore, the antibodies generated by genetic immunization are usually of superior quality with regard to specificity, affinity and recognizing the native protein.
  • 克隆多克隆
  • 同种型IgG
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab22093 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/1000. Predicted molecular weight: 44 kDa.

This antibody has been tested in Western blot against an E.coli lysate containing the partial recombinant fusion protein used as an immunogen. We have no data on detection of endogenous protein.

靶标

  • 功能SIII, also known as elongin, is a general transcription elongation factor that increases the RNA polymerase II transcription elongation past template-encoded arresting sites. Subunit A is transcriptionally active and its transcription activity is strongly enhanced by binding to the dimeric complex of the SIII regulatory subunits B and C (elongin BC complex).
  • 序列相似性Contains 1 F-box domain.
    Contains 1 TFIIS N-terminal domain.
  • 结构域The elongin BC complex binding domain is also known as BC-box with the consensus [APST]-L-x(3)-C-x(3)-[AILV].
  • 细胞定位Nucleus.
  • Information by UniProt
  • 数据库链接
    • 别名
      • Ela1 antibody
      • Elo A antibody
      • EloA antibody
      • ELOA1_HUMAN antibody
      • Elongin 110 kDa subunit antibody
      • Elongin-A antibody
      • ElonginA antibody
      • RNA polymerase II transcription factor SIII subunit A1 antibody
      • SIII antibody
      • SIII p110 antibody
      • TCEB 3 antibody
      • Tceb3 antibody
      • TCEB3A antibody
      • Transcription elongation factor B (SIII) polypeptide 3 (110kD) antibody
      • Transcription elongation factor B (SIII) polypeptide 3 antibody
      • Transcription elongation factor B alpha subunit antibody
      • Transcription elongation factor B polypeptide 3 antibody
      see all

    Anti-Elongin A antibody (ab22093)参考文献

    ab22093 has not yet been referenced specifically in any publications.

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