ELMO2 overexpression 293T裂解液(全细胞) (ab94207)

概述

  • 产品名称
    ELMO2 overexpression 293T裂解液(全细胞)
  • 描述
    ELMO2 overexpression裂解液
  • 常规说明
    ab94207 is a 293T cell transfected lysate in which Human ELMO2 has been transiently over-expressed using a pCMV-ELMO2 plasmid. The lysate is provided in 1 x Sample Buffer. Note: For more details about how the transfected lysate was prepared view preparation notes
  • 经测试应用
    适用于: WBmore details

性能

  • 形式
    Liquid
  • 存放说明
    Shipped on dry ice. Upon delivery aliquot and store at -20ºC. Avoid freeze / thaw cycles.
  • 存储溶液
    pH: 6.80
    Constituent: 100% 1x Sample Buffer
  • Concentration information loading...
  • 纯度
    Whole Cell Lysate
  • 研究领域
  • 背景
    Function: Involved in cytoskeletal rearrangements required for phagocytosis of apoptotic cells and cell motility. Acts in assocation with DOCK1 and CRK. Was initially proposed to be required in complex with DOCK1 to activate Rac Rho small GTPases. May enhance the guanine nucleotide exchange factor (GEF) activity of DOCK1. Tissue specificity: Widely expressed, with a higher expression in skeletal muscle, kidney and placenta. Similarity: Contains 1 ELMO domain. Contains 1 PH domain.

应用

Our Abpromise guarantee covers the use of ab94207 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use at an assay dependent dilution.

图片

  • ab94207 at 15µg/lane on an SDS-PAGE gel.
  • All lanes : Anti-ELMO2 antibody (ab57557) at 1/500 dilution

    Lane 1 : ELMO2 overexpression 293T lysate (whole cell) (ab94207)
    Lane 2 : 293T non-transfected lysate

    Lysates/proteins at 25 µg per lane.

    Secondary
    Goat Anti-mouse IgG (H and L) HRP conjugated at 1/2500 dilution

文献

ab94207 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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