ELMO2 overexpression 293T裂解液(全细胞) (ab94207)

概述

  • 产品名称ELMO2 overexpression 293T裂解液(全细胞)
  • 描述
    ELMO2 overexpression裂解液
  • 常规说明ab94207 is a 293T cell transfected lysate in which Human ELMO2 has been transiently over-expressed using a pCMV-ELMO2 plasmid. The lysate is provided in 1 x Sample Buffer. Note: For more details about how the transfected lysate was prepared view preparation notes
  • 经测试应用适用于: WBmore details

性能

  • 背景Function: Involved in cytoskeletal rearrangements required for phagocytosis of apoptotic cells and cell motility. Acts in assocation with DOCK1 and CRK. Was initially proposed to be required in complex with DOCK1 to activate Rac Rho small GTPases. May enhance the guanine nucleotide exchange factor (GEF) activity of DOCK1. Tissue specificity: Widely expressed, with a higher expression in skeletal muscle, kidney and placenta. Similarity: Contains 1 ELMO domain. Contains 1 PH domain.

应用

Our Abpromise guarantee covers the use of ab94207 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use at an assay dependent dilution.

ELMO2 overexpression 293T lysate (whole cell) 图像

  • ab94207 at 15µg/lane on an SDS-PAGE gel.
  • All lanes : Anti-ELMO2 antibody (ab57557) at 1/500 dilution

    Lane 1 : ELMO2 overexpression 293T lysate (whole cell) (ab94207)
    Lane 2 : 293T non-transfected lysate

    Lysates/proteins at 25 µg per lane.

    Secondary
    Goat Anti-mouse IgG (H and L) HRP conjugated at 1/2500 dilution

ELMO2 overexpression 293T lysate (whole cell) (ab94207)参考文献

ab94207 has not yet been referenced specifically in any publications.

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