重组Anti-ELMO1 + ELMO2抗体[EPR13567] (ab181234)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR13567] to ELMO1 + ELMO2
- Suitable for: WB
- Reacts with: Human
Related conjugates and formulations
概述
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产品名称
Anti-ELMO1 + ELMO2抗体[EPR13567] -
描述
兔单克隆抗体[EPR13567] to ELMO1 + ELMO2 -
宿主
Rabbit -
经测试应用
适用于: WBmore details -
种属反应性
与反应: Human
预测可用于: Mouse, Rat -
免疫原
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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阳性对照
- Jurkat, HeLa cells, Human fetal brain lysates.
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常规说明
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
存储溶液
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading...
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纯度
Tissue culture supernatant -
克隆
单克隆 -
克隆编号
EPR13567 -
同种型
IgG -
研究领域
相关产品
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Alternative Versions
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Isotype control
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Positive Controls
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Recombinant Protein
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Related Products
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab181234于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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WB |
1/1000 - 1/2000. Predicted molecular weight: 83 kDa.
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说明 |
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WB
1/1000 - 1/2000. Predicted molecular weight: 83 kDa. |
靶标
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细胞定位
ELMO1: Cytoplasm. Cell membrane. Translocation to plasma membrane seems to be mediated by DOCK1 and CRK. ELMO2: Cytoplasm. Cytoplasm > cytosol. Membrane. -
数据库链接
- Entrez Gene: 63916 Human
- Entrez Gene: 9844 Human
- Entrez Gene: 140579 Mouse
- Entrez Gene: 140580 Mouse
- Entrez Gene: 361251 Rat
- Entrez Gene: 362271 Rat
- Omim: 606420 Human
- Omim: 606421 Human
see all
图片
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All lanes : Anti-ELMO1 + ELMO2 antibody [EPR13567] (ab181234) at 1/2000 dilution
Lane 1 : ELMO2 Human Recombinant Protein
Lane 2 : ELMO1 Human Recombinant Protein
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Predicted band size: 83 kDa
Exposure time: 20 secondsBlocking and diluting buffer and concentration: 5% NFDM/TBST.
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All lanes : Anti-ELMO1 + ELMO2 antibody [EPR13567] (ab181234) at 1/2000 dilution
Lane 1 : Jurkat cell lysate
Lane 2 : HeLa cell lysate
Lane 3 : Human fetal brain lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
Predicted band size: 83 kDa
数据表及文件
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SDS download
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Datasheet download
Certificate of Compliance
文献 (2)
ab181234 被引用在 2 文献中.
- Ho E et al. Integrin-linked kinase and ELMO2 modulate recycling endosomes in keratinocytes. Biochim Biophys Acta 1863:2892-2904 (2016). Mouse . PubMed: 27627840
- Jackson BC et al. An ELMO2-RhoG-ILK network modulates microtubule dynamics. Mol Biol Cell 26:2712-25 (2015). Mouse . PubMed: 25995380