重组HRP Anti-ELMO1抗体[EPR12919] (ab204386)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- HRP Rabbit monoclonal [EPR12919] to ELMO1
- Suitable for: WB
- Reacts with: Human
- Conjugation: HRP
Related conjugates and formulations
概述
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产品名称
HRP Anti-ELMO1抗体[EPR12919]
参阅全部 ELMO1 一抗 -
描述
HRP兔单克隆抗体[EPR12919] to ELMO1 -
宿主
Rabbit -
偶联物
HRP -
经测试应用
适用于: WBmore details -
种属反应性
与反应: Human
预测可用于: Mouse, Rat -
免疫原
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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阳性对照
- WB: Human placenta tissue and Jurkat whole cell lysates.
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常规说明
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. Store In the Dark. -
存储溶液
pH: 7.40
Preservative: 0.1% Proclin 300 Solution
Constituents: PBS, 30% Glycerol (glycerin, glycerine), 1% BSA -
Concentration information loading...
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纯度
Protein A purified -
克隆
单克隆 -
克隆编号
EPR12919 -
同种型
IgG -
研究领域
相关产品
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Alternative Versions
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Isotype control
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Positive Controls
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Recombinant Protein
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Related Products
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab204386于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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WB |
1/5000. Detects a band of approximately 84 kDa (predicted molecular weight: 84 kDa).
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说明 |
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WB
1/5000. Detects a band of approximately 84 kDa (predicted molecular weight: 84 kDa). |
靶标
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功能
Involved in cytoskeletal rearrangements required for phagocytosis of apoptotic cells and cell motility. Acts in assocation with DOCK1 and CRK. Was initially proposed to be required in complex with DOCK1 to activate Rac Rho small GTPases. May enhance the guanine nucleotide exchange factor (GEF) activity of DOCK1. -
组织特异性
Widely expressed, with a higher expression in the spleen and placenta. -
序列相似性
Contains 1 ELMO domain.
Contains 1 PH domain. -
翻译后修饰
Phosphorylated by HCK. -
细胞定位
Cytoplasm. Cell membrane. Translocation to plasma membrane seems to be mediated by DOCK1 and CRK. - Information by UniProt
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数据库链接
- Entrez Gene: 9844 Human
- Entrez Gene: 140580 Mouse
- Entrez Gene: 361251 Rat
- Omim: 606420 Human
- SwissProt: Q92556 Human
- SwissProt: Q8BPU7 Mouse
- Unigene: 656638 Human
- Unigene: 342392 Mouse
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别名
- CED 12 antibody
- Ced 12 homolog 1 antibody
- Ced 12 homolog antibody
see all
图片
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All lanes : HRP Anti-ELMO1 antibody [EPR12919] (ab204386) at 1/5000 dilution
Lane 1 : Human placenta tissue lysate - total protein (ab29745)
Lane 2 : Jurkat (Human T cell lymphoblast-like cell line) Whole Cell Lysate
Lysates/proteins at 10 µg per lane.
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 84 kDa
Observed band size: 84 kDa
Exposure time: 2 minutesThis blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 2% Bovine Serum Albumin before being incubated with ab204386 overnight at 4°C. Antibody binding was visualised using ECL development solution ab133406.
数据表及文件
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SDS download
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Datasheet download
文献 (0)
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