Reactive against mammalian transfected lysate.
Full length protein, corresponding to amino acids 1-398 of Human ELL3
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
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Protein A purified
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in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/500 - 1/1000. Detects a band of approximately 60 kDa (predicted molecular weight: 45 kDa).
ELL3 is a testis-specific RNA polymerase II elongation factor which increases the catalytic rate of transcription elongation. ELL3 is approximately 50% similar to both ELL and ELL2.
Elongation Factor RNA Polymerase II-Like 3 antibody
RNA polymerase II elongation factor ELL3 antibody
Western blot - ELL3 antibody (ab67415)
All lanes :
Anti-ELL3 antibody (ab67415) at 1/500 dilution
Lane 1 :
ELL3 transfected 293T lysate
Lane 2 :
Non transfected 293T lysate
Lysates/proteins at 25 µg per lane.
Goat Anti-Mouse IgG (H&L)-HRP Conjugate at 1/2500 dilution
Predicted band size :
Observed band size :
43.78 kDa (
why is the actual band size different from the predicted?
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