概述

  • 产品名称
  • 描述
    小鼠多克隆抗体to ELL3
  • 特异性
    Reactive against mammalian transfected lysate.
  • 经测试应用
    适用于: WBmore details
  • 种属反应性
    与反应: Human
  • 免疫原

    Full length protein, corresponding to amino acids 1-398 of Human ELL3

  • 阳性对照
    • Transfected 293T lysate

性能

应用

Our Abpromise guarantee covers the use of ab67415 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/500 - 1/1000. Detects a band of approximately 60 kDa (predicted molecular weight: 45 kDa).

靶标

  • 相关性
    ELL3 is a testis-specific RNA polymerase II elongation factor which increases the catalytic rate of transcription elongation. ELL3 is approximately 50% similar to both ELL and ELL2.
  • 细胞定位
    Nuclear
  • 数据库链接
  • 别名
    • Elongation Factor RNA Polymerase II-Like 3 antibody
    • RNA polymerase II elongation factor ELL3 antibody

图片

  • All lanes : Anti-ELL3 antibody (ab67415) at 1/500 dilution

    Lane 1 : ELL3 transfected 293T lysate
    Lane 2 : Non transfected 293T lysate

    Lysates/proteins at 25 µg per lane.

    Secondary
    Goat Anti-Mouse IgG (H&L)-HRP Conjugate at 1/2500 dilution

    Predicted band size : 45 kDa
    Observed band size : 43.78 kDa (why is the actual band size different from the predicted?)

实验方案

文献

This product has been referenced in:
  • Ahn HJ  et al. Ell3 stabilizes p53 following CDDP treatment via its effects on ubiquitin-dependent and -independent proteasomal degradation pathways in breast cancer cells. Oncotarget 6:44523-37 (2015). WB . Read more (PubMed: 26540344) »

See 1 Publication for this product

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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