The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Direct ELISA: 1/25000.
WB: 1/1000. Predicted molecular weight: 47 kDa.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
Forms a ternary complex with the serum response factor (SRF). Requires DNA-bound SRF for ternary complex formation and makes extensive DNA contacts to the 5'side of SRF, but does not bind DNA autonomously.
Belongs to the ETS family. Contains 1 ETS DNA-binding domain.