Anti-ELK4抗体(ab86002)
Key features and details
- Rabbit polyclonal to ELK4
- Suitable for: WB
- Reacts with: Recombinant fragment
- Isotype: IgG
概述
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产品名称
Anti-ELK4抗体 -
描述
兔多克隆抗体to ELK4 -
宿主
Rabbit -
经测试应用
适用于: WBmore details -
种属反应性
与反应: Recombinant fragment -
免疫原
Three synthetic peptides derived from human ELK4, conjugated to KLH.
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
存储溶液
Preservative: 0.01% Thimerosal (merthiolate) -
Concentration information loading...
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纯度
Whole antiserum -
纯化说明
Crude serum, final bleed -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab86002于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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WB |
1/1000. Predicted molecular weight: 47 kDa.
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说明 |
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WB
1/1000. Predicted molecular weight: 47 kDa. |
靶标
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功能
Forms a ternary complex with the serum response factor (SRF). Requires DNA-bound SRF for ternary complex formation and makes extensive DNA contacts to the 5'side of SRF, but does not bind DNA autonomously. -
序列相似性
Belongs to the ETS family.
Contains 1 ETS DNA-binding domain. -
细胞定位
Nucleus. - Information by UniProt
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别名
- Elk4 antibody
- ELK4_HUMAN antibody
- ETS domain containing protein Elk4 antibody
see all
图片
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All lanes : Anti-ELK4 antibody (ab86002) at 1/1000 dilution (in 5% non-fat milk-PBST solution incubated for 60 minutes)
Lane 1 : uninduced culture of E. coli (plus anti thio at 1/5000 dilution)
Lane 2 : induced culture of E. coli (plus anti thio at 1/5000 dilution)
Secondary
All lanes : anti-Rabbit IgG HRP conjugated incubated for 60 minutes at 1/1000 dilution
Predicted band size: 47 kDa
Observed band size: 61 kDa why is the actual band size different from the predicted?
Gel concentration: 10%
The actual band molecular weight is the result of the target protein ELK4 (47 kDa) attached to a tag (14 kDa).
数据表及文件
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SDS download
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Datasheet download
文献 (1)
ab86002 被引用在 1 文献中.
- Mao X et al. ELK4-mediated lncRNA SNHG22 promotes gastric cancer progression through interacting with EZH2 and regulating miR-200c-3p/Notch1 axis. Cell Death Dis 12:957 (2021). PubMed: 34663788