概述

  • 产品名称Anti-ELK4抗体
    参阅全部 ELK4 一抗
  • 描述
    兔多克隆抗体to ELK4
  • 经测试应用适用于: WB, ELISAmore details
  • 种属反应性
    与反应: Human
  • 免疫原

    A region withinynthetic peptide: VAPLSPARLQ GANTLFQFPS VLNSHGPFTL SGLDGPSTPG PFSPDLQKT, corresponding to C terminal amino acids 383-431 of Human ELK4

  • 阳性对照
    • Jurkat cell lysate.

性能

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应用

Our Abpromise guarantee covers the use of ab49244 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use a concentration of 2.5 µg/ml. Detects a band of approximately 41 kDa (predicted molecular weight: 47 kDa). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
ELISA Use at an assay dependent concentration.

ELISA titre using peptide based assay: 1/62500.

靶标

  • 功能Forms a ternary complex with the serum response factor (SRF). Requires DNA-bound SRF for ternary complex formation and makes extensive DNA contacts to the 5'side of SRF, but does not bind DNA autonomously.
  • 序列相似性Belongs to the ETS family.
    Contains 1 ETS DNA-binding domain.
  • 细胞定位Nucleus.
  • Information by UniProt
  • 数据库链接
  • 别名
    • Elk4 antibody
    • ELK4_HUMAN antibody
    • ETS domain containing protein Elk4 antibody
    • ETS domain protein antibody
    • ETS domain-containing protein Elk-4 antibody
    • SAP-1 antibody
    • SAP1 antibody
    • Serum response factor accessory protein 1 antibody
    • SRF accessory protein 1 antibody
    • TCF1 antibody
    see all

Anti-ELK4 antibody 图像

  • Anti-ELK4 antibody (ab49244) at 2.5 µg/ml + Jurkat cell lysate at 10 µg with 5% skim milk in PBS.

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size : 47 kDa
    Observed band size : 41 kDa (why is the actual band size different from the predicted?)

Anti-ELK4 antibody (ab49244)参考文献

ab49244 has not yet been referenced specifically in any publications.

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