概述

  • 产品名称Anti-ELK4抗体
    参阅全部 ELK4 一抗
  • 描述
    小鼠多克隆抗体to ELK4
  • 经测试应用适用于: WBmore details
  • 种属反应性
    与反应: Human
  • 免疫原

    Synthetic peptide: AEKKSPQEPT PSVIKFVTTP SKKPPVEPVA ATISIGPSIS PSSEETIQAL ETLVSPKLPS LEAPTSASNV MTAFATTPPI SSIPPLQEPP RTPSPPLSSH , corresponding to amino acids 176-276 of Human ELK4

  • 常规说明


    This antibody was raised by a genetic immunization technique. Genetic immunization can be used to generate antibodies by directly delivering antigen-coding DNA into the animal, rather than injecting a protein or peptide (Tang et al. PubMed: 1545867; Chambers and Johnston PubMed 12910245; Barry and Johnston PubMed: 9234514). The animal's cells produce the protein, which stimulates the animal's immune system to produce antibodies against that particular protein. A vector coding for a partial fusion protein was used for genetic immunisation of a mouse and the resulting serum was tested in Western blot against an E.coli lysate containing that partial fusion protein. Genetic immunization offers enormous advantages over the traditional protein-based immunization method. DNA is faster, cheaper and easier to produce and can be produced by standard techniques readily amenable to automation. Furthermore, the antibodies generated by genetic immunization are usually of superior quality with regard to specificity, affinity and recognizing the native protein.

性能

  • 形式Liquid
  • 存放说明Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • 存储溶液Preservative: None
    Constituents: 50% Glycerol, Whole serum
  • 纯度Whole antiserum
  • Primary antibody说明This antibody was raised by a genetic immunization technique. Genetic immunization can be used to generate antibodies by directly delivering antigen-coding DNA into the animal, rather than injecting a protein or peptide (Tang et al. PubMed: 1545867; Chambers and Johnston PubMed 12910245; Barry and Johnston PubMed: 9234514). The animal's cells produce the protein, which stimulates the animal's immune system to produce antibodies against that particular protein. A vector coding for a partial fusion protein was used for genetic immunisation of a mouse and the resulting serum was tested in Western blot against an E.coli lysate containing that partial fusion protein. Genetic immunization offers enormous advantages over the traditional protein-based immunization method. DNA is faster, cheaper and easier to produce and can be produced by standard techniques readily amenable to automation. Furthermore, the antibodies generated by genetic immunization are usually of superior quality with regard to specificity, affinity and recognizing the native protein.
  • 克隆多克隆
  • 同种型IgG
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab43741 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/1000. Detects a band of approximately 38 kDa (predicted molecular weight: 47 kDa). This antibody has been tested in Western blot against an E.coli lysate containing the partial recombinant fusion protein used as an immunogen. We have no data on detection of endogenous protein.

靶标

  • 功能Forms a ternary complex with the serum response factor (SRF). Requires DNA-bound SRF for ternary complex formation and makes extensive DNA contacts to the 5'side of SRF, but does not bind DNA autonomously.
  • 序列相似性Belongs to the ETS family.
    Contains 1 ETS DNA-binding domain.
  • 细胞定位Nucleus.
  • Information by UniProt
  • 数据库链接
  • 别名
    • Elk4 antibody
    • ELK4_HUMAN antibody
    • ETS domain containing protein Elk4 antibody
    • ETS domain protein antibody
    • ETS domain-containing protein Elk-4 antibody
    • SAP-1 antibody
    • SAP1 antibody
    • Serum response factor accessory protein 1 antibody
    • SRF accessory protein 1 antibody
    • TCF1 antibody
    see all

Anti-ELK4 antibody 图像

  • All lanes : Anti-ELK4 antibody (ab43741) at 1/1000 dilution

    Lane 1 : a total protein extract from E coli with 50ng to 100 ng of a GST fusion protein of an irrelevant antigen
    Lane 2 : a total protein extract from E coli with 50ng to 500ng of the antigen (GST-antigen fusion protein)

    Lysates/proteins at 20 µg per lane.

    Secondary
    Rabbit anti-mouse IgG + IgM, (H+L) conjugated to Horseradish peroxidase at 1/5000 dilution

    Predicted band size : 47 kDa

Anti-ELK4 antibody (ab43741)参考文献

ab43741 has not yet been referenced specifically in any publications.

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