概述

  • 产品名称
  • 描述
    兔多克隆抗体to ELK4
  • 宿主
    Rabbit
  • 特异性
    ab33075 recognises ETS domain protein Elk-4 (ELK4).
  • 经测试应用
    适用于: WB, ELISAmore details
  • 种属反应性
    与反应: Human
    预测可用于: Mouse, Rat, Rabbit, Horse, Chicken, Guinea pig, Cow, Cat, Dog, Pig
  • 免疫原

    Synthetic peptide within Human ELK4 aa 382-431 (C terminal). The exact sequence is proprietary.
    Sequence:

    PVAPLSPARLQGANTLFQFPSVLNSHGPFTLSGLDGPSTPGPFSPDLQKT


    Database link: P28324

  • 阳性对照
    • Jurkat cell lysate

性能

应用

Our Abpromise guarantee covers the use of ab33075 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use a concentration of 2.5 µg/ml. Predicted molecular weight: 40 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
ELISA 1/62500.

靶标

图片

  • Anti-ELK4 antibody (ab33075) at 2.5 µg/ml + Jurkat cell lysate at 10 µg with 5% skimmed milk in PBS

    Secondary
    HRP conjugated anti-Rabbit IgG diluted in 1: 50,000 - 100,000

    Predicted band size: 40 kDa
    Observed band size: 40 kDa



    Gel concentration: 12%

实验方案

文献

ab33075 has not yet been referenced specifically in any publications.

客户评价及客户问答

There are currently no Customer reviews or Questions for ab33075.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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