概述

  • 产品名称
  • 描述
    兔多克隆抗体to ELK3
  • 经测试应用
    适用于: WBmore details
  • 种属反应性
    与反应: Mouse, Human
    预测可用于: Rat, Chicken, Cow, Dog
  • 免疫原

    Synthetic peptide corresponding to a region within internal sequence amino acids 251-300 (LPSEHRSLFL EAACHDSDSL EPLNLSSGSK TKSPSLPPKA KKPKGLEISA) of Human ELK3 (NP_005221)

  • 阳性对照
    • Fetal heart lysate

性能

应用

Our Abpromise guarantee covers the use of ab83186 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 44 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.

靶标

图片

  • Anti-ELK3 antibody (ab83186) at 1 µg/ml + Human fetal heart lysate at 10 µg

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size : 44 kDa
    Observed band size : 44 kDa
    Additional bands at : 28 kDa. We are unsure as to the identity of these extra bands.

实验方案

文献

ab83186 has not yet been referenced specifically in any publications.

客户评价及客户问答

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Mouse Cell lysate - whole cell (NIH 3T3 cells)
Loading amount
15 µg
Specification
NIH 3T3 cells
Gel Running Conditions
Non-reduced Denaturing (4-12% gradient Bis-Tris gel)
Blocking step
Milk as blocking agent for 16 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 4°C
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提交于 Jan 22 2010

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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