Anti-ELK1 (phospho T417)抗体(ab28817)

概述

  • 产品名称Anti-ELK1 (phospho T417)抗体
    参阅全部 ELK1 一抗
  • 描述
    兔多克隆抗体to ELK1 (phospho T417)
  • 特异性ab28817 recognises Phospho-ELK-1(Thr417).
  • 经测试应用适用于: ELISA, IP, IHC-Pmore details
  • 种属反应性
    与反应: Human
    预测可用于: Mouse
  • 免疫原

    Synthetic phospho-peptide derived from human ELK-1 around the phosphorylation site of Threonine 417.

  • 阳性对照
    • Breast carcinoma.

性能

应用

Our Abpromise guarantee covers the use of ab28817 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ELISA 1/10000.
IP Use at an assay dependent concentration.
IHC-P Use at an assay dependent concentration.

靶标

  • 功能Stimulates transcription. Binds to purine-rich DNA sequences. Can form a ternary complex with the serum response factor and the ETS and SRF motifs of the fos serum response element.
  • 组织特异性Lung and testis.
  • 序列相似性Belongs to the ETS family.
    Contains 1 ETS DNA-binding domain.
  • 翻译后修饰Sumoylation represses transcriptional activator activity as it results in recruitment of HDAC2 to target gene promoters which leads to decreased histone acetylation and reduced transactivator activity. It also regulates nuclear retention.
    On mitogenic stimulation, phosphorylated on C-terminal serine and threonine residues by MAPK1. Ser-383 and Ser-389 are the preferred sites for MAPK1. In vitro, phosphorylation by MAPK1 potentiates ternary complex formation with the serum responses factors, SRE and SRF. Phosphorylation leads to loss of sumoylation and restores transcriptional activator activity.
  • 细胞定位Nucleus.
  • Information by UniProt
  • 数据库链接
  • 别名
    • ELK 1 antibody
    • Elk1 antibody
    • ELK1 member of ETS oncogene family antibody
    • ELK1 protein antibody
    • ELK1_HUMAN antibody
    • ELK2 member of ETS oncogene family antibody
    • ETS domain containing protein Elk 1 antibody
    • ETS domain containing protein Elk1 antibody
    • ETS domain protein Elk1 antibody
    • ETS domain-containing protein Elk-1 antibody
    • ETS like gene 1 antibody
    • Member of ETS oncogene family antibody
    • Oncogene Elk1 antibody
    • Tyrosine kinase (ELK1) oncogene antibody
    see all

Anti-ELK1 (phospho T417) antibody 图像

  • Immunohistochemical analysis of paraffin-embedded breast carcinoma. Left: Using Phospho-ELK-1(Thr417) Antibody (ab28817); Right: The same antibody preincubated with synthesized phosphopeptide.

Anti-ELK1 (phospho T417) antibody (ab28817)参考文献

ab28817 has not yet been referenced specifically in any publications.

Product Wall

Abcam has not validated the combination of species/application used in this Abreview.
Application Western blot
Loading amount 30 µg
Gel Running Conditions Reduced Denaturing (15%)
Sample Human Cell lysate - whole cell (A431 Cells)
Specification A431 Cells
Treatment 30uM etoposide for 30 hrs
Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
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提交于 Jul 17 2013

Thank you for your enquiry and your interest in our products.

I regret to inform that you that the blocking peptide (neither the immunizing peptides) are commercially available.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"