Anti-ELK1 (phospho S389)抗体(ab28818)

概述

  • 产品名称Anti-ELK1 (phospho S389)抗体
    参阅全部 ELK1 一抗
  • 描述
    兔多克隆抗体to ELK1 (phospho S389)
  • 特异性ab28818 recognises phospho-ELK-1(Ser389).
  • 经测试应用适用于: WB, ELISA, IHC-P, IPmore details
  • 种属反应性
    与反应: Mouse, Human
  • 免疫原

    Synthetic phospho-peptide derived from human ELK-1 around the phosphorylation site of Serine 389.

  • 阳性对照
    • Breast carcinoma.

性能

应用

Our Abpromise guarantee covers the use of ab28818 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/500 - 1/1000. Predicted molecular weight: 45 kDa.
ELISA 1/10000.
IHC-P Use at an assay dependent concentration.
IP Use at an assay dependent concentration.

靶标

  • 功能Stimulates transcription. Binds to purine-rich DNA sequences. Can form a ternary complex with the serum response factor and the ETS and SRF motifs of the fos serum response element.
  • 组织特异性Lung and testis.
  • 序列相似性Belongs to the ETS family.
    Contains 1 ETS DNA-binding domain.
  • 翻译后修饰Sumoylation represses transcriptional activator activity as it results in recruitment of HDAC2 to target gene promoters which leads to decreased histone acetylation and reduced transactivator activity. It also regulates nuclear retention.
    On mitogenic stimulation, phosphorylated on C-terminal serine and threonine residues by MAPK1. Ser-383 and Ser-389 are the preferred sites for MAPK1. In vitro, phosphorylation by MAPK1 potentiates ternary complex formation with the serum responses factors, SRE and SRF. Phosphorylation leads to loss of sumoylation and restores transcriptional activator activity.
  • 细胞定位Nucleus.
  • Information by UniProt
  • 数据库链接
  • 别名
    • ELK 1 antibody
    • Elk1 antibody
    • ELK1 member of ETS oncogene family antibody
    • ELK1 protein antibody
    • ELK1_HUMAN antibody
    • ELK2 member of ETS oncogene family antibody
    • ETS domain containing protein Elk 1 antibody
    • ETS domain containing protein Elk1 antibody
    • ETS domain protein Elk1 antibody
    • ETS domain-containing protein Elk-1 antibody
    • ETS like gene 1 antibody
    • Member of ETS oncogene family antibody
    • Oncogene Elk1 antibody
    • Tyrosine kinase (ELK1) oncogene antibody
    see all

Anti-ELK1 (phospho S389) antibody 图像

  • Immunohistochemical analysis of paraffin-embedded breast carcinoma. Left: Using Phospho-ELK-1(Ser389) ab28818; Right: The same antibody preincubated with synthesized phosphopeptide.
  • ab28818 staining ELK1 (phospho S389) in human bladder transitional cell carcinoma tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde; antigen retrieval was by heat mediation with a citrate buffer. Samples were incubated with primary antibody (1/50) for 1 hour at 20°C. An undiluted HRP-conjugated Mouse anti-rabbit polyclonal was used as the secondary antibody.

    See Abreview

Anti-ELK1 (phospho S389) antibody (ab28818)参考文献

ab28818 has not yet been referenced specifically in any publications.

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Thank you for your enquiry and your interest in our products.

I regret to inform that you that the blocking peptide (neither the immunizing peptides) are commercially available.

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Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Human Tissue sections (human bladder transitional cell carcinoma)
Specification human bladder transitional cell carcinoma
Fixative Formaldehyde
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: citrate
Permeabilization No
Username

Dr. Imran Ahmad

Verified customer

提交于 Nov 22 2010

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"