RabMAb

Anti-ELK1抗体[E277] - ChIP Grade (ab32106)

概述

  • 产品名称Anti-ELK1抗体[E277] - ChIP Grade
    参阅全部 ELK1 一抗
  • 描述
    兔单克隆抗体[E277] to ELK1 - ChIP Grade
  • 特异性This antibody does not cross react with other ETS family members
  • 经测试应用适用于: WB, IHC-P, ICC, IP, ChIPmore details
    不适用于: Flow Cyt
  • 种属反应性
    与反应: Mouse, Rat, Human
  • 免疫原

    Synthetic peptide corresponding to residues in C terminus of human ELK1.

  • 表位ab32106 reacts with an epitope located in the C terminal region of ELK1.
  • 阳性对照
    • Human breast carcinoma
  • 常规说明

    This product is a recombinant rabbit monoclonal antibody.

    Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.

性能

应用

Our Abpromise guarantee covers the use of ab32106 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/500. Detects a band of approximately 62 kDa (predicted molecular weight: 45 kDa).
IHC-P 1/50.
ICC 1/100.
IP 1/80.
ChIP Use at an assay dependent concentration. PubMed: 22589737
  • 应用说明Is unsuitable for Flow Cyt.
  • 靶标

    • 功能Stimulates transcription. Binds to purine-rich DNA sequences. Can form a ternary complex with the serum response factor and the ETS and SRF motifs of the fos serum response element.
    • 组织特异性Lung and testis.
    • 序列相似性Belongs to the ETS family.
      Contains 1 ETS DNA-binding domain.
    • 翻译后修饰Sumoylation represses transcriptional activator activity as it results in recruitment of HDAC2 to target gene promoters which leads to decreased histone acetylation and reduced transactivator activity. It also regulates nuclear retention.
      On mitogenic stimulation, phosphorylated on C-terminal serine and threonine residues by MAPK1. Ser-383 and Ser-389 are the preferred sites for MAPK1. In vitro, phosphorylation by MAPK1 potentiates ternary complex formation with the serum responses factors, SRE and SRF. Phosphorylation leads to loss of sumoylation and restores transcriptional activator activity.
    • 细胞定位Nucleus.
    • Information by UniProt
    • 数据库链接
    • 别名
      • ELK 1 antibody
      • Elk1 antibody
      • ELK1 member of ETS oncogene family antibody
      • ELK1 protein antibody
      • ELK1_HUMAN antibody
      • ELK2 member of ETS oncogene family antibody
      • ETS domain containing protein Elk 1 antibody
      • ETS domain containing protein Elk1 antibody
      • ETS domain protein Elk1 antibody
      • ETS domain-containing protein Elk-1 antibody
      • ETS like gene 1 antibody
      • Member of ETS oncogene family antibody
      • Oncogene Elk1 antibody
      • Tyrosine kinase (ELK1) oncogene antibody
      see all

    Anti-ELK1 antibody [E277] - ChIP Grade 图像

    • Anti-ELK1 antibody [E277] - ChIP Grade (ab32106) at 1/500 dilution

      Predicted band size : 45 kDa
      Observed band size : 62 kDa (why is the actual band size different from the predicted?)
    • Immunohistochemical analysis of paraffin embedded human breast carcinoma using ab32106 at a dilution of 1/50

    Anti-ELK1 antibody [E277] - ChIP Grade (ab32106)参考文献

    This product has been referenced in:
    • Aguilar-Martinez E  et al. The ubiquitin ligase UBE3A dampens ERK pathway signalling in HPV E6 transformed HeLa cells. PLoS One 10:e0119366 (2015). Read more (PubMed: 25815718) »
    • Sillivan SE  et al. ELK1 transcription factor linked to dysregulated striatal mu opioid receptor signaling network and OPRM1 polymorphism in human heroin abusers. Biol Psychiatry 74:511-9 (2013). IP ; Human . Read more (PubMed: 23702428) »

    See all 12 Publications for this product

    Product Wall

    The concentration of this lot is 0.2180 mg/ml

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"