重组
RabMAb

Anti-ELK1抗体[E277] - ChIP Grade (ab32106)

概述

  • 产品名称
    Anti-ELK1抗体[E277] - ChIP Grade
    参阅全部 ELK1 一抗
  • 描述
    兔单克隆抗体[E277] to ELK1 - ChIP Grade
  • 特异性
    This antibody does not cross react with other ETS family members
  • 经测试应用
    适用于: WB, IHC-P, ICC, IP, Flow Cyt, ChIPmore details
  • 种属反应性
    与反应: Mouse, Rat, Human
  • 免疫原

    Synthetic peptide corresponding to residues in C terminus of human ELK1.

  • 表位
    ab32106 reacts with an epitope located in the C terminal region of ELK1.
  • 阳性对照
    • Human breast carcinoma
  • 常规说明

    This product is a recombinant rabbit monoclonal antibody.

    Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.

性能

应用

Our Abpromise guarantee covers the use of ab32106 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/500. Detects a band of approximately 62 kDa (predicted molecular weight: 45 kDa).
IHC-P 1/50.
ICC 1/100.
IP 1/80.
Flow Cyt 1/20.
ChIP Use at an assay dependent concentration. PubMed: 22589737

靶标

  • 功能
    Stimulates transcription. Binds to purine-rich DNA sequences. Can form a ternary complex with the serum response factor and the ETS and SRF motifs of the fos serum response element.
  • 组织特异性
    Lung and testis.
  • 序列相似性
    Belongs to the ETS family.
    Contains 1 ETS DNA-binding domain.
  • 翻译后修饰
    Sumoylation represses transcriptional activator activity as it results in recruitment of HDAC2 to target gene promoters which leads to decreased histone acetylation and reduced transactivator activity. It also regulates nuclear retention.
    On mitogenic stimulation, phosphorylated on C-terminal serine and threonine residues by MAPK1. Ser-383 and Ser-389 are the preferred sites for MAPK1. In vitro, phosphorylation by MAPK1 potentiates ternary complex formation with the serum responses factors, SRE and SRF. Phosphorylation leads to loss of sumoylation and restores transcriptional activator activity.
  • 细胞定位
    Nucleus.
  • Information by UniProt
  • 数据库链接
  • 别名
    • ELK 1 antibody
    • Elk1 antibody
    • ELK1 member of ETS oncogene family antibody
    • ELK1 protein antibody
    • ELK1, ETS transcription factor antibody
    • ELK1_HUMAN antibody
    • ELK2 member of ETS oncogene family antibody
    • ETS domain containing protein Elk 1 antibody
    • ETS domain containing protein Elk1 antibody
    • ETS domain protein Elk1 antibody
    • ETS domain-containing protein Elk-1 antibody
    • ETS like gene 1 antibody
    • Member of ETS oncogene family antibody
    • Oncogene Elk1 antibody
    • Tyrosine kinase (ELK1) oncogene antibody
    see all

Anti-ELK1 antibody [E277] - ChIP Grade 图像

  • Anti-ELK1 antibody [E277] - ChIP Grade (ab32106) at 1/500 dilution

    Predicted band size : 45 kDa
    Observed band size : 62 kDa (why is the actual band size different from the predicted?)
  • Immunohistochemical analysis of paraffin embedded human breast carcinoma using ab32106 at a dilution of 1/50
  • Flow Cytometry analysis of HeLa (human cervix adenocarcinoma) cells labeling ELK1 with unpurified ab32106 at 1/20 dilution (10ug/mL) (red). Cells were fixed with 4% paraformaldehyde and permeabilised with 90% methanol. A Goat anti rabbit IgG (Alexa Fluor® 488) at 1/2000 dilution was used as the secondary antibody. Rabbit monoclonal IgG (Black) was used as the isotype control, cells without incubation with primary antibody and secondary antibody (Blue) were used as the unlabeled control.

Anti-ELK1 antibody [E277] - ChIP Grade (ab32106)参考文献

This product has been referenced in:
  • Shuang T  et al. NF-?B1, c-Rel, and ELK1 inhibit miR-134 expression leading to TAB1 upregulation in paclitaxel-resistant human ovarian cancer. Oncotarget 8:24853-24868 (2017). ChIP . Read more (PubMed: 28206956) »
  • Rosati R  et al. The Amino-terminal Domain of the Androgen Receptor Co-opts Extracellular Signal-regulated Kinase (ERK) Docking Sites in ELK1 Protein to Induce Sustained Gene Activation That Supports Prostate Cancer Cell Growth. J Biol Chem 291:25983-25998 (2016). Read more (PubMed: 27793987) »

See all 15 Publications for this product

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The concentration of this lot is 0.2180 mg/ml

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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