概述

  • 产品名称
  • 描述
    兔多克隆抗体to ELK1
  • 经测试应用
    适用于: WBmore details
  • 种属反应性
    与反应: Mouse, Human
  • 免疫原

    Synthetic peptide:

    WSTLSPIAPRSPAK-C

    , with a C-terminal Cysteine, corresponding to amino acids 379-382 of Human ELK1.

  • 常规说明
    If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. Working dilution samples should be discarded if not used within 12 hours.

性能

应用

Our Abpromise guarantee covers the use of ab11847 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB
  • 应用说明
    WB: 1/1000. This concentration is recommended using Elk1 fusion protein. Predicted molecular weight: 45 kDa. This antibody detects total Elk1, independent of phosphorylation state.

    Not tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • 靶标

    • 功能
      Stimulates transcription. Binds to purine-rich DNA sequences. Can form a ternary complex with the serum response factor and the ETS and SRF motifs of the fos serum response element.
    • 组织特异性
      Lung and testis.
    • 序列相似性
      Belongs to the ETS family.
      Contains 1 ETS DNA-binding domain.
    • 翻译后修饰
      Sumoylation represses transcriptional activator activity as it results in recruitment of HDAC2 to target gene promoters which leads to decreased histone acetylation and reduced transactivator activity. It also regulates nuclear retention.
      On mitogenic stimulation, phosphorylated on C-terminal serine and threonine residues by MAPK1. Ser-383 and Ser-389 are the preferred sites for MAPK1. In vitro, phosphorylation by MAPK1 potentiates ternary complex formation with the serum responses factors, SRE and SRF. Phosphorylation leads to loss of sumoylation and restores transcriptional activator activity.
    • 细胞定位
      Nucleus.
    • Information by UniProt
    • 数据库链接
    • 别名
      • ELK 1 antibody
      • Elk1 antibody
      • ELK1 member of ETS oncogene family antibody
      • ELK1 protein antibody
      • ELK1, ETS transcription factor antibody
      • ELK1_HUMAN antibody
      • ELK2 member of ETS oncogene family antibody
      • ETS domain containing protein Elk 1 antibody
      • ETS domain containing protein Elk1 antibody
      • ETS domain protein Elk1 antibody
      • ETS domain-containing protein Elk-1 antibody
      • ETS like gene 1 antibody
      • Member of ETS oncogene family antibody
      • Oncogene Elk1 antibody
      • Tyrosine kinase (ELK1) oncogene antibody
      see all

    实验方案

    文献

    This product has been referenced in:
    • Al-Sadi R  et al. TNF-a Modulation of Intestinal Epithelial Tight Junction Barrier Is Regulated by ERK1/2 Activation of Elk-1. Am J Pathol 183:1871-84 (2013). Read more (PubMed: 24121020) »

    See 1 Publication for this product

    客户评价及客户问答

    Thank you for your enquiry. The concentration of ab11847 is 75 ug/ml, and the concentration of ab11848 is 20 ug/ml. If you have any more questions, please contact us again.

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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