Anti-ELK1抗体[7E10D5] (ab47712)

概述

  • 产品名称Anti-ELK1抗体[7E10D5]
    参阅全部 ELK1 一抗
  • 描述
    小鼠单克隆抗体[7E10D5] to ELK1
  • 特异性This antibody is specific for ELK1.
  • 经测试应用适用于: WB, ELISAmore details
  • 种属反应性
    与反应: Human
  • 免疫原

    Ni-NTA purified truncated recombinant ELK1 expressed in E. Coli strain BL21 (DE3)

  • 阳性对照
    • K562 cell lysate

性能

相关产品

应用

Our Abpromise guarantee covers the use of ab47712 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/500 - 1/1000. Detects a band of approximately 40, 77 kDa (predicted molecular weight: 45 kDa).
ELISA 1/10000.

靶标

  • 功能Stimulates transcription. Binds to purine-rich DNA sequences. Can form a ternary complex with the serum response factor and the ETS and SRF motifs of the fos serum response element.
  • 组织特异性Lung and testis.
  • 序列相似性Belongs to the ETS family.
    Contains 1 ETS DNA-binding domain.
  • 翻译后修饰Sumoylation represses transcriptional activator activity as it results in recruitment of HDAC2 to target gene promoters which leads to decreased histone acetylation and reduced transactivator activity. It also regulates nuclear retention.
    On mitogenic stimulation, phosphorylated on C-terminal serine and threonine residues by MAPK1. Ser-383 and Ser-389 are the preferred sites for MAPK1. In vitro, phosphorylation by MAPK1 potentiates ternary complex formation with the serum responses factors, SRE and SRF. Phosphorylation leads to loss of sumoylation and restores transcriptional activator activity.
  • 细胞定位Nucleus.
  • Information by UniProt
  • 数据库链接
  • 别名
    • ELK 1 antibody
    • Elk1 antibody
    • ELK1 member of ETS oncogene family antibody
    • ELK1 protein antibody
    • ELK1_HUMAN antibody
    • ELK2 member of ETS oncogene family antibody
    • ETS domain containing protein Elk 1 antibody
    • ETS domain containing protein Elk1 antibody
    • ETS domain protein Elk1 antibody
    • ETS domain-containing protein Elk-1 antibody
    • ETS like gene 1 antibody
    • Member of ETS oncogene family antibody
    • Oncogene Elk1 antibody
    • Tyrosine kinase (ELK1) oncogene antibody
    see all

Anti-ELK1 antibody [7E10D5] 图像

  • All lanes : Anti-ELK1 antibody [7E10D5] (ab47712) at 1/500 dilution

    Lane 1 : truncated recombinant ELK1
    Lane 2 : K562 cell lysate


    Predicted band size : 45 kDa
    Observed band size : 40,77 kDa (why is the actual band size different from the predicted?)

Anti-ELK1 antibody [7E10D5] (ab47712)参考文献

ab47712 has not yet been referenced specifically in any publications.

Product Wall

There are currently no Abreviews or Questions for ab47712.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"